The pathogenesis of murine cytomegalovirus ocular infection. Anterior chamber inoculation

J F Bale Jr; M E O'Neil; B Lyon; S Perlman

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The pathogenesis of murine cytomegalovirus ocular infection. Anterior chamber inoculation

Journal article

Open access

Peer reviewed

The pathogenesis of murine cytomegalovirus ocular infection. Anterior chamber inoculation

J F Bale Jr, M E O'Neil, B Lyon and S Perlman

Investigative ophthalmology & visual science, Vol.31(8), pp.1575-1581

08/1990

PMID: 2167300

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Abstract

To investigate the pathogenesis of ocular cytomegalovirus infections, 3-week-old BALB/c mice were inoculated with 10(4) plaque-forming units of murine cytomegalovirus (MCMV) by the right anterior chamber and studied sequentially with the use of virus assays and in situ nucleic acid hybridization methods. During acute infection, MCMV was recovered from the right vitreous, lens, cornea, retina/choroid, and optic nerve. Titers of MCMV exceeded 10(3) per ml of homogenate on days 4 and 7 after inoculation. With the use of biotinylated MCMV DNA probes, MCMV nucleic acids were detected in and adjacent to cells of the iris and ciliary body and occasionally within inflammatory lesions of the cornea. During chronic infection, MCMV was recovered, with the use of co-cultivation or explant methods, from ocular tissues of occasional mice inoculated with MCMV 1 yr earlier. Infectious MCMV was also recovered from the ocular homogenates of a group of mice immunosuppressed with antilymphocyte serum and cortisone. These studies indicate that cells of the uveal tract are permissive for MCMV and suggest that intrinsic persistence or latency of cytomegalovirus in ocular tissues could contribute to the pathogenesis of ocular infections in immunosuppressed hosts.

Nucleic Acid Hybridization

Cytomegalovirus - genetics

Cytomegalovirus - isolation & purification

DNA Probes

Eye - pathology

Acute Disease

Anterior Chamber - microbiology

Cytomegalovirus Infections - microbiology

Spleen - microbiology

Animals

Leukocytes - microbiology

Cytomegalovirus Infections - etiology

Cytomegalovirus Infections - pathology

Mice

Mice, Inbred BALB C

Salivary Glands - microbiology

Chronic Disease

Cytomegalovirus - physiology

Virus Replication - physiology

Eye - microbiology

Details

Title: Subtitle: The pathogenesis of murine cytomegalovirus ocular infection. Anterior chamber inoculation
Creators: J F Bale Jr
M E O'Neil
B Lyon
S Perlman
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.31(8), pp.1575-1581
PMID: 2167300
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Grant note: EY06802 / NEI NIH HHS
Language: English
Date published: 08/1990
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777353302771

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