Journal article
The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription
Cell (Cambridge), Vol.67(1), pp.155-167
10/04/1991
DOI: 10.1016/0092-8674(91)90579-N
PMID: 1833069
Abstract
The NTRC protein of enteric bacteria is an enhancer-binding protein that activates transcription in response to limitation of combined nitrogen. NTRC activates transcription by catalyzing formation of open complexes by RNA polymerase (θ54 holoenzyme form) in an ATP-dependent reaction. To catalyze open complex formation, NTRC must be phosphorylated. We show that phosphorylated NTRC has an ATPase activity, and we present biochemical and genetic evidence that NTRC must hydrolyze ATP to catalyze open complex formation. It is likely that all activators of θ54 holoenzyme have an ATPase activity.
Details
- Title: Subtitle
- The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription
- Creators
- David S WeissJacques BatutKarl E KloseJohn KeenerSydney Kustu
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.67(1), pp.155-167
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0092-8674(91)90579-N
- PMID
- 1833069
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 10/04/1991
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984002393802771
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