The pro-apoptotic gene Bax is required for the death of ectopic primordial germ cells during their migration in the mouse embryo

James Stallock; Kathy Molyneaux; Kyle Schaible; C Michael Knudson; Christopher Wylie

doi:10.1242/dev.00898

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The pro-apoptotic gene Bax is required for the death of ectopic primordial germ cells during their migration in the mouse embryo

Journal article

Open access

Peer reviewed

The pro-apoptotic gene Bax is required for the death of ectopic primordial germ cells during their migration in the mouse embryo

James Stallock, Kathy Molyneaux, Kyle Schaible, C Michael Knudson and Christopher Wylie

Development (Cambridge), Vol.130(26), pp.6589-6597

12/2003

DOI: 10.1242/dev.00898

PMID: 14660547

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Abstract

In the mouse embryo, significant numbers of primordial germ cells (PGCs) fail to migrate correctly to the genital ridges early in organogenesis. These usually die in ectopic locations. In humans, 50% of pediatric germ line tumors arise outside the gonads, and these are thought to arise from PGCs that fail to die in ectopic locations. We show that the pro-apoptotic gene Bax, previously shown to be required for germ cell death during later stages of their differentiation in the gonads, is also expressed during germ cell migration, and is required for the normal death of germ cells left in ectopic locations during and after germ cell migration. In addition, we show that Bax is downstream of the known cell survival signaling interaction mediated by the Steel factor/Kit ligand/receptor interaction. Together, these observations identify the major mechanism that removes ectopic germ cells from the embryo at early stages.

Green Fluorescent Proteins

Embryonic and Fetal Development - genetics

Cell Survival

Gene Expression Regulation, Developmental - genetics

Apoptosis - genetics

Male

Mice, Transgenic

Proto-Oncogene Proteins - genetics

bcl-2-Associated X Protein

Choristoma - embryology

Animals

Ovum - physiology

Cell Death

Female

Luminescent Proteins - genetics

Spermatozoa - physiology

Mice

Choristoma - genetics

Proto-Oncogene Proteins c-bcl-2

Organ Culture Techniques

Cell Movement

Details

Title: Subtitle: The pro-apoptotic gene Bax is required for the death of ectopic primordial germ cells during their migration in the mouse embryo
Creators: James Stallock - Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, USA
Kathy Molyneaux
Kyle Schaible
C Michael Knudson
Christopher Wylie
Resource Type: Journal article
Publication Details: Development (Cambridge), Vol.130(26), pp.6589-6597
DOI: 10.1242/dev.00898
PMID: 14660547
NLM abbreviation: Development
ISSN: 0950-1991
eISSN: 1477-9129
Publisher: England
Grant note: R01 HD33440 / NICHD NIH HHS 1 F32 HD008678-01 / NICHD NIH HHS 1R01CA88967 / NCI NIH HHS
Language: English
Date published: 12/2003
Academic Unit: Pathology; Radiation Oncology
Record Identifier: 9984046818502771

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