Journal article
The prolonged life-span of alveolar macrophages
American journal of respiratory cell and molecular biology, Vol.38(4), pp.380-385
04/01/2008
DOI: 10.1165/rcmb.2007-0224RC
PMCID: PMC2274942
PMID: 18192503
Abstract
To further examine the half-life of alveolar macrophages, chimeric CD 45.2 mice were generated through bone marrow transplantation of donor CD 45.1 cells. Before administration of donor cells, recipient mice were divided into two cohorts: the first cohort received total body irradiation; the second cohort also received irradiation-however, the thorax, head, and upper extremities were shielded with lead. Flow cytometric analysis was then performed on blood, peritoneal, and bronchoalveolar lavage cells over time to quantify engraftment. The data generated for the unshielded cohort of mice revealed a macrophage half-life of 30 days. In the shielded cohort, however, we found that by 8 months there was negligible replacement of recipient alveolar macrophages by donor cells, despite reconstitution of the blood and peritoneum by donor bone marrow. Consistent with these findings, the mean fluorescent intensity of alveolar macrophages remained stable over a 4-week period after in vivo PKH26 dye loading. Together, these data show that previous alveolar macrophage half-life studies were confounded by the fact that they did not account for the toxic effects of irradiation conditioning regimens, and demonstrate that the bone marrow does not significantly contribute to the alveolar macrophage compartment during steady-state conditions.
Details
- Title: Subtitle
- The prolonged life-span of alveolar macrophages
- Creators
- Jaime Murphy - Boston UniversityRoss Summer - Boston UniversityAndrew A. Wilson - Boston UniversityDarrell N. Kotton - Boston UniversityAlan Fine - Boston University
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.38(4), pp.380-385
- Publisher
- Amer Thoracic Soc
- DOI
- 10.1165/rcmb.2007-0224RC
- PMID
- 18192503
- PMCID
- PMC2274942
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Number of pages
- 6
- Grant note
- 5R21 HL086414; R21 HL082778; K08 HL077138 / NHLBI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) K08HL077138 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Language
- English
- Date published
- 04/01/2008
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984694745102771
Metrics
2 Record Views