The reaction of superoxide, formate radical, and hydrated electron with transferrin and its model compound, Fe(III)-ethylenediamine-N,N'-bis[2-(2-hydroxyphenyl)acetic acid] as studied by pulse radiolysis

Garry R Buettner

doi:10.1016/S0021-9258(18)45308-2

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The reaction of superoxide, formate radical, and hydrated electron with transferrin and its model compound, Fe(III)-ethylenediamine-N,N'-bis[2-(2-hydroxyphenyl)acetic acid] as studied by pulse radiolysis

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The reaction of superoxide, formate radical, and hydrated electron with transferrin and its model compound, Fe(III)-ethylenediamine-N,N'-bis[2-(2-hydroxyphenyl)acetic acid] as studied by pulse radiolysis

Garry R Buettner

The Journal of biological chemistry, Vol.262(25), pp.11995-11998

1987

DOI: 10.1016/S0021-9258(18)45308-2

PMID: 3040725

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Abstract

Transferrin and the transferrin model compound Fe(III)-EHPG (Fe(III)-ethylenediamine-N,N'-bis[2-(2-hydroxyphenyl)acetic acid] were found not to react with superoxide, as pulse radiolysis and kinetic spectroscopy revealed no transient species and no bleaching of the 465-nm absorption. However, transferrin was found to react with the formate radical, CO-.2, and the hydrated electron, e-aq, with second-order rate constants of 3.8 × 10(8) and 1.1 × 10(10) M-1 S-1, respectively. These reactions produced a transient species (lambda max = 420 nm) which subsequently decayed by a second-order process. However, no reduction of the Fe(III) in transferrin was detected. Fe(III)-EHPG was also found to react with CO-.2 and e-aq, k = 7.3 × 10(6) and 1.1 × 10(9) M-1 S-1, respectively. The reactions of CO-.2 and e-aq with Fe(III)-EHPG resulted in no transient species but rather in reduction of the iron. These results are consistent with the inability of transferrin and Fe(III)-EHPG to catalyze the Haber-Weiss reaction.

Fundamental and applied biological sciences. Psychology

Proteins

Biological and medical sciences

Analytical, structural and metabolic biochemistry

Metalloproteins

Other metalloproteins

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Title: Subtitle: The reaction of superoxide, formate radical, and hydrated electron with transferrin and its model compound, Fe(III)-ethylenediamine-N,N'-bis[2-(2-hydroxyphenyl)acetic acid] as studied by pulse radiolysis
Creators: Garry R Buettner - GSF forschungszentr., inst. strahlenbiologie, Neuherberg 8042, Germany
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.262(25), pp.11995-11998
DOI: 10.1016/S0021-9258(18)45308-2
PMID: 3040725
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology; Bethesda, MD
Language: English
Date published: 1987
Academic Unit: Radiation Oncology
Record Identifier: 9984046920902771

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