Journal article
The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
Scientific reports, Vol.11(1), pp.21627-21627
11/03/2021
DOI: 10.1038/s41598-021-01088-7
PMCID: PMC8566492
PMID: 34732805
Abstract
Numerous studies have shown that cg05575921 methylation decreases in response to smoking. However, secondary to methodological issues, the magnitude and dose dependency of that response is as of yet unclear. This lack of certainty is a barrier to the use of DNA methylation clinically to assess and monitor smoking status. To better define this relationship, we conducted a joint analysis of methylation sensitive PCR digital (MSdPCR) assessments of cg05575921 methylation in whole blood and/or saliva DNA to smoking using samples from 421 smokers and 423 biochemically confirmed non-smokers from 4 previously published studies. We found that cg05575921 methylation manifested a curvilinear dose dependent decrease in response to increasing cigarette consumption. In whole blood DNA, the Receiver Operating Characteristic (ROC) Area Under the Curve (AUC) of cg05575921 methylation for predicting daily smoking status was 0.98. In saliva DNA, the gross AUC was 0.91 with correction for cellular heterogeneity improving the AUC to 0.94. Methylation status was significantly associated with the Fagerstrom Test for Nicotine Dependence score, but with significant sampling heterogeneity. We conclude that MSdPCR assessments of cg05575921 methylation are a potentially powerful, clinically implementable tool for the assessment and management of smoking.
Details
- Title: Subtitle
- The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
- Creators
- Kelsey Dawes - University of IowaAllan Andersen - University of IowaRachel Reimer - Des Moines UniversityJames A. Mills - University of IowaEric Hoffman - University of IowaJeffrey D. Long - University of IowaShelly Miller - Behavioral Diagnostics (United States)Robert Philibert - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.11(1), pp.21627-21627
- DOI
- 10.1038/s41598-021-01088-7
- PMID
- 34732805
- PMCID
- PMC8566492
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Publisher
- NATURE PORTFOLIO
- Number of pages
- 12
- Grant note
- Smoke Free World Foundation 5R01HL130883; R44CA213507; R44AA022041 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 11/03/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Psychiatry; Iowa Neuroscience Institute; Biostatistics; Internal Medicine
- Record Identifier
- 9984280844502771
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