Journal article
The rise of CLAVATA: evidence for CLAVATA3 and WOX signaling in the fern gametophyte
The Plant journal : for cell and molecular biology, Vol.121(2), e17207
01/2025
DOI: 10.1111/tpj.17207
PMCID: PMC11771683
PMID: 39673734
Appears in UI Libraries Support Open Access
Abstract
CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are 12-13 amino acid-long peptides that serve as positional signals in plants. The core CLE signaling module consists of a CLE peptide and a leucine-rich repeat receptor-like kinase, but in flowering plants, WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors are also incorporated to form negative feedback loops that regulate stem cell maintenance in the shoot and root. It is not known when WOX genes were co-opted into CLE signaling pathways, only that mosses and liverworts do not require WOX for CLE-regulated stem cell activities. We identified 11 CLE-encoding genes in the Ceratopteris genome, including one (CrCLV3) most similar to shoot meristem CLE peptide CLAVATA3. We performed the first functional characterization of a fern CLE using techniques including RNAi knockdown and synthetic peptide dosage. We found that CrCLV3 promotes cell proliferation and stem cell identity in the gametophyte meristem. Importantly, we provide evidence for CrCLV3 regulation of the WOX gene CrWOXA during the developmental stage when female gametangium formation begins. These discoveries open a new avenue for CLE peptide research in the fern and clarify the evolutionary timeline of CLE-WOX signaling in land plants.
Details
- Title: Subtitle
- The rise of CLAVATA: evidence for CLAVATA3 and WOX signaling in the fern gametophyte
- Creators
- Kelley A Renninger - University of IowaRebekah M Yarvis - University of IowaChristopher E Youngstrom - University of IowaChi-Lien Cheng - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Plant journal : for cell and molecular biology, Vol.121(2), e17207
- DOI
- 10.1111/tpj.17207
- PMID
- 39673734
- PMCID
- PMC11771683
- NLM abbreviation
- Plant J
- ISSN
- 0960-7412
- eISSN
- 1365-313X
- Publisher
- Wiley
- Grant note
- 1555487 / National Science Foundation Kollros Graduate Fund Avis Cone Undergraduate Research Fellowship 2423834 / National Science Foundation UI Graduate College Summer Fellowship University of Iowa (UI) Graduate College Post-Comps Fellowship Linda and Rick Maxson Undergraduate Research Award Ballard and Seashore Dissertation Fellowship
- Language
- English
- Electronic publication date
- 12/14/2024
- Date published
- 01/2025
- Academic Unit
- Biology
- Record Identifier
- 9984757684902771
Metrics
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