The rkp-3 gene region of Sinorhizobium meliloti Rm41 contains strain-specific genes that determine K antigen structure

Ernö Kiss; Attila Kereszt; Fatime Barta; Samuel Stephens; Bradley L Reuhs; Adam Kondorosi; Péter Putnoky

doi:10.1094/MPMI.2001.14.12.1395

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The rkp-3 gene region of Sinorhizobium meliloti Rm41 contains strain-specific genes that determine K antigen structure

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The rkp-3 gene region of Sinorhizobium meliloti Rm41 contains strain-specific genes that determine K antigen structure

Ernö Kiss, Attila Kereszt, Fatime Barta, Samuel Stephens, Bradley L Reuhs, Adam Kondorosi and Péter Putnoky

Molecular plant-microbe interactions, Vol.14(12), pp.1395-1403

2001

DOI: 10.1094/MPMI.2001.14.12.1395

PMID: 11768534

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Abstract

The rkp-3 region is indispensable for capsular polysaccharide (K antigen) synthesis in Sinorhizobium meliloti Rm41. Strain Rm41 produces a K antigen of strain-specific structure, designated as the KR5 antigen. The data in this report show that the rkp-3 gene region comprises 10 open reading frames involved in bacterial polysaccharide synthesis and export. The predicted amino acid sequences for the rkpL-Q gene products are homologous to enzymes involved in the production of specific sugar moieties, while the putative products of the rkpRST genes show a high degree of similarity to proteins required for transporting polysaccharides to the cell surface. Southern analysis experiments using gene-specific probes suggest that genes involved in the synthesis of the precursor sugars are unique in strain Rm41, whereas sequences coding for export proteins are widely distributed among Sinorhizobium species. Mutations in the rkpL-Q genes result in a modified K antigen pattern and impaired symbiotic capabilities. On this basis, we suggest that these genes are required for the production of the KR5 antigen that is necessary for S. meliloti Rm41 exoB (AK631) - alfalfa (Medicago sativa) symbiosis.

Symbiosis

Agronomy. Soil science and plant productions

Biological and medical sciences

Economic plant physiology

Fundamental and applied biological sciences. Psychology

Parasitism and symbiosis

Plant physiology and development

Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)

Details

Title: Subtitle: The rkp-3 gene region of Sinorhizobium meliloti Rm41 contains strain-specific genes that determine K antigen structure
Creators: Ernö Kiss - Hungarian Academy of Sciences
Attila Kereszt - Hungarian Academy of Sciences
Fatime Barta - Hungarian Academy of Sciences
Samuel Stephens - Hungarian Academy of Sciences
Bradley L Reuhs - Hungarian Academy of Sciences
Adam Kondorosi - Hungarian Academy of Sciences
Péter Putnoky - Hungarian Academy of Sciences
Resource Type: Journal article
Publication Details: Molecular plant-microbe interactions, Vol.14(12), pp.1395-1403
DOI: 10.1094/MPMI.2001.14.12.1395
PMID: 11768534
NLM abbreviation: Mol Plant Microbe Interact
ISSN: 0894-0282
eISSN: 1943-7706
Publisher: APS Press
Language: English
Date published: 2001
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984359869802771

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