Journal article
The role of CC and CXC chemokines in cardiac allograft rejection in rats
Experimental and molecular pathology, Vol.78(3), pp.171-176
2005
DOI: 10.1016/j.yexmp.2005.01.002
PMID: 15924868
Abstract
Acute cellular rejection is due in part to an upregulation of chemokine genes, resulting in eventual cell-mediated cytotoxicity. The role of chemokines in acute cardiac allograft rejection is not fully characterized presently. These studies compared the patterns of expression for multiple chemokines in rodent cardiac allograft rejection. Allogeneic transplants were performed from Brown–Norway donors to Lewis recipients. Survival studies utilized daily administration of neutralizing antisera to MCP-1, CINC, and MIP-1α. Patterns of mRNA and protein expression were determined by Northern blots and immunohistochemistry. Allogeneic controls rejected at mean of 6.5 days. Neutralization of MCP-1 (10.8 days,
P < 0.001) and MIP-1α (7.5 days,
P = 0.004) function, but not CINC (6.2 days,
P > 0.05), significantly prolonged allograft survival. Message expression for the beta chemokines studied were increased by day 2 and continued to increase until day 6 just before rejection, while CINC levels did not change as dramatically after day 2. Chemokine protein levels mirrored mRNA patterns by IHC analysis. MCP-1 and MIP-1α appear to play regulatory roles in cardiac allograft rejection, while CINC is expressed, but not functional, in injury development. Beta chemokine activity should be studied further in hope of developing more targeted immunosuppression, or identifying specific chemokines that may be useful for immunosurveillance purposes.
Details
- Title: Subtitle
- The role of CC and CXC chemokines in cardiac allograft rejection in rats
- Creators
- Alexander S Farivar - University of Washington Medical CenterBrendan C Mackinnon-Patterson - University of Washington Medical CenterAnton S McCourtie - University of Washington Medical CenterPeter A Ward - University of Michigan Medical SchoolMichael S Mulligan - University of Washington Medical Center
- Resource Type
- Journal article
- Publication Details
- Experimental and molecular pathology, Vol.78(3), pp.171-176
- DOI
- 10.1016/j.yexmp.2005.01.002
- PMID
- 15924868
- NLM abbreviation
- Exp Mol Pathol
- ISSN
- 0014-4800
- eISSN
- 1096-0945
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2005
- Academic Unit
- Orthopedics and Rehabilitation
- Record Identifier
- 9984040459002771
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