Journal article
The role of phosphatidylinositol-3 kinase in vanadate-promoted S phase entry
Molecular and cellular biochemistry, Vol.255(1), pp.239-245
01/2004
DOI: 10.1023/B:MCBI.0000007279.28280.e5
PMID: 14971664
Abstract
Phosphatidylinositil-3 kinase (PI3K) is a heterodimer of catalytic and regulatory subunits. It is involved in various signaling pathways and key functions of the cells. The present study investigated the role of PI3K in vanadate-induced alteration in cell cycle regulation in C141 mouse epidermal cells. Vanadate caused a time- and dose-dependent increase in PI3K activity and phosphorylation of p70 S6 kinase (p70S6K) at Thr421/Ser424 and Thr389 sites. The phosphorylation at these sites was inhibited by PI3K inhibitor, LY294002, and p70S6K mutation. Vanadate promoted S phase entry and this promotion was inhibited by LY294002 and rapmycin, a p70S6K inhibitor. Vanadate-induced enhancement in S phase entry was also inhibited in transfection with dominant negative p70S6K mutant cells. The results obtained show that vanadate is able to increase PI3K activity through phosphorylation. PI3K activated p70S6K, which phosphated protein S6, and promoted S phase entry.
Details
- Title: Subtitle
- The role of phosphatidylinositol-3 kinase in vanadate-promoted S phase entry
- Creators
- Zhuo Zhang - West Virginia UniversityNing Gao - West Virginia UniversityHengyun He - West Virginia UniversityChuanshu Huang - New York UniversityBing-hua Jiang - West Virginia UniversityJia Luo - West Virginia UniversityXianglin Shi - National Institute for Occupational Safety and Health
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biochemistry, Vol.255(1), pp.239-245
- Publisher
- Kluwer Academic Publishers
- DOI
- 10.1023/B:MCBI.0000007279.28280.e5
- PMID
- 14971664
- ISSN
- 0300-8177
- eISSN
- 1573-4919
- Language
- English
- Date published
- 01/2004
- Academic Unit
- Pathology; Radiation Oncology
- Record Identifier
- 9984201253702771
Metrics
16 Record Views