Journal article
The role of retinol dehydrogenase 10 in the cone visual cycle
Scientific reports, Vol.7(1), pp.2390-13
05/24/2017
DOI: 10.1038/s41598-017-02549-8
PMCID: PMC5443843
PMID: 28539612
Abstract
Pigment regeneration is critical for the function of cone photoreceptors in bright and rapidly-changing light conditions. This process is facilitated by the recently-characterized retina visual cycle, in which Muller cells recycle spent all-trans-retinol visual chromophore back to 11-cis-retinol. This 11-cis-retinol is oxidized selectively in cones to the 11-cis-retinal used for pigment regeneration. However, the enzyme responsible for the oxidation of 11-cis-retinol remains unknown. Here, we sought to determine whether retinol dehydrogenase 10 (RDH10), upregulated in rod/cone hybrid retinas and expressed abundantly in Muller cells, is the enzyme that drives this reaction. We created mice lacking RDH10 either in cone photoreceptors, Muller cells, or the entire retina. In vivo electroretinography and transretinal recordings revealed normal cone photoresponses in all RDH10-deficient mouse lines. Notably, their cone-driven dark adaptation both in vivo and in isolated retina was unaffected, indicating that RDH10 is not required for the function of the retina visual cycle. We also generated transgenic mice expressing RDH10 ectopically in rod cells. However, rod dark adaptation was unaffected by the expression of RDH10 and transgenic rods were unable to use cis-retinol for pigment regeneration. We conclude that RDH10 is not the dominant retina 11-cis-RDH, leaving its primary function in the retina unknown.
Details
- Title: Subtitle
- The role of retinol dehydrogenase 10 in the cone visual cycle
- Creators
- Yunlu Xue - Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USAShinya Sato - Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USADavid Razafsky - Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USABhubanananda Sahu - Case Western Reserve UniversitySusan Q. Shen - Washington University in St. LouisChloe Potter - Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USALisa L. Sandell - University of LouisvilleJoseph C. Corbo - Washington University in St. LouisKrzysztof Palczewski - Case Western Reserve UniversityAkiko Maeda - Case Western Reserve UniversityDidier Hodzic - Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USAVladimir J. Kefalov - Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USA
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.7(1), pp.2390-13
- Publisher
- NATURE PORTFOLIO
- DOI
- 10.1038/s41598-017-02549-8
- PMID
- 28539612
- PMCID
- PMC5443843
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Number of pages
- 13
- Grant note
- EY019312; EY025696; EY009339; EY024864; EY022658; EY022632; EY024958; EY025196; EY026672; EY013360; DE025960; EY002687 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Research to Prevent Blindness; Research to Prevent Blindness (RPB)
- Language
- English
- Date published
- 05/24/2017
- Academic Unit
- Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984618519402771
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