Journal article
The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis
Developmental biology, Vol.356(2), pp.398-410
2011
DOI: 10.1016/j.ydbio.2011.05.666
PMCID: PMC3143288
PMID: 21651900
Abstract
The Drosophila Suppressor of Hairy wing [Su(Hw)] insulator protein has an essential role in the development of the female germline. Here we investigate the function of Su(Hw) in the ovary. We show that Su(Hw) is universally expressed in somatic cells, while germ cell expression is dynamic. Robust levels accumulate in post-mitotic germ cells, where Su(Hw) localization is limited to chromosomes within nurse cells, the specialized cells that support oocyte growth. Although loss of Su(Hw) causes global defects in nurse cell chromosome structure, we demonstrate that these architectural changes are not responsible for the block in oogenesis. Connections between the fertility and insulator functions of Su(Hw) were investigated through studies of the two
gypsy insulator proteins, Modifier of (mdg4)67.2 (Mod67.2) and Centrosomal Protein of 190
kDa (CP190). Accumulation of these proteins is distinct from Su(Hw), with Mod67.2 and CP190 showing uniform expression in all cells during early stages of oogenesis that diminishes in later stages. Although Mod67.2 and CP190 extensively co-localize with Su(Hw) on nurse cell chromosomes, neither protein is required for nurse cell chromosome development or oocyte production. These data indicate that while the
gypsy insulator function requires both Mod67.2 and CP190, these proteins are not essential for oogenesis. These studies represent the first molecular investigations of Su(Hw) function in the germline, which uncover distinct requirements for Su(Hw) insulator and ovary functions.
► The insulator and fertility functions of Suppressor of Hairy-wing are different. ► Sterility of
su(Hw) mutants is not due to altered ribosome biogenesis. ► Architectural changes in nurse cell chromosomes are not required for oogenesis. ► Mod67.2 and CP190 are not essential for Drosophila oogenesis.
Details
- Title: Subtitle
- The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis
- Creators
- Ryan M Baxley - Molecular Biology Program, University of Iowa, College of Medicine, Iowa City, IA 52242, USAAlexey A Soshnev - Molecular Biology Program, University of Iowa, College of Medicine, Iowa City, IA 52242, USADmitry E Koryakov - Department of Molecular and Cellular Biology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, RussiaIgor F Zhimulev - Department of Molecular and Cellular Biology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, RussiaPamela K Geyer - Molecular Biology Program, University of Iowa, College of Medicine, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.356(2), pp.398-410
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.ydbio.2011.05.666
- PMID
- 21651900
- PMCID
- PMC3143288
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Grant note
- name: National Institute of Health, award: GM42539; name: National Institute of Health, award: T32073610; name: Russian Foundation for Basic Research, award: 09-04-00437, 6.4, SB RAS N 37, ROSNAUKA 02.740.11.0099
- Language
- English
- Date published
- 2011
- Academic Unit
- Obstetrics and Gynecology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024406002771
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