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The spectrum of antibacterial activity of human amniotic fluid determined by scanning electron microscopy
Journal article   Peer reviewed

The spectrum of antibacterial activity of human amniotic fluid determined by scanning electron microscopy

Bryan Larsen, Patrick Schlievert and Rudolph Galask
American journal of obstetrics and gynecology, Vol.119(7), pp.895-903
08/01/1974
DOI: 10.1016/0002-9378(74)90004-0
PMID: 4366790

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Abstract

Bacterial cells exposed to fresh human amniotic fluid examined by scanning-beam electron microscopy consistently showed aberrant morphology. This study included Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium perfringens, group B Streptococcus, and Staphylococcus aureus and revealed that all species studied became elongated upon exposure to amniotic fluid. Elongation of cocci resulted in the formation of rod-shaped forms. It is proposed that the elongated forms may be the result of bacterial growth with concomitant blockade of cell division. Additional cellular alterations observed included apparent spheroplast formation seen with Pseudomonas aeruginosa and damage to cellular surfaces of Clostridium perfringes; however, these effects were not seen in the other species studied.

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