Journal article
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)
Genome research, Vol.14(10B), pp.2121-2127
10/2004
DOI: 10.1101/gr.2596504
PMCID: PMC528928
PMID: 15489334
Abstract
The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.
Details
- Title: Subtitle
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)
- Creators
- Daniela S GerhardLukas WagnerElise A FeingoldCarolyn M ShenmenLynette H GrouseGreg SchulerSteven L KleinSusan OldRebekah RasoolyPeter GoodMark GuyerAllison M PeckJeffery G DergeDavid LipmanFrancis S CollinsWonhee JangSteven SherryMike FeoloLeonie MisquittaEduardo LeeKirill RotmistrovskySusan F GreenhutCarl F SchaeferKenneth BuetowTom I BonnerDavid HausslerJim KentMark KiekhausTerry FureyMichael BrentChrista PrangeKirsten SchreiberNarayan K BhatRalph F HopkinsFlorence HsieTom DriscollM Bento SoaresTom L CasavantTodd E ScheetzMichael J BrownsteinTed B UsdinShiraki ToshiyukiPiero CarninciYulan PiaoDawood B DudekulaMinoru S H KoKoichi KawakamiYutaka SuzukiSumio SuganoC E GruberMark R SmithBlake SimmonsTroy MooreRichard WatermanStephen L JohnsonYijun RuanChia Lin WeiS MathavanPreethi H GunaratneJiaqian WuAngela M GarciaStephen W HulykEdwin FuhAnna SneedCarla KowisAnne HodgsonDonna M MuznyJohn McPhersonRichard A GibbsJessica FaheyErin HeltonMark KettemanAnuradha MadanStephanie RodriguesAmy SanchezMichelle WhitingAnup MadariAlice C YoungKeith D WetherbySteven J GranitePeggy N KwongCharles P BrinkleyRussell L PearsonGerard G BouffardRobert W BlakeslyEric D GreenMark C DicksonAlex C RodriguezJane GrimwoodJeremy SchmutzRichard M MyersYaron S N ButterfieldMalachi GriffithObi L GriffithMartin I KrzywinskiNancy LiaoRyan MorinRyan MorrinDiana PalmquistAnca S PetrescuUrsula SkalskaDuane E SmailusJeff M StottAngelique SchnerchJacqueline E ScheinSteven J M JonesRobert A HoltAgnes BarossMarco A MarraSandra CliftonKathryn A MakowskiStephanie BosakJoel MalekNicole ShapiroYe Yuan - Baylor College of MedicineMGC Project Team
- Resource Type
- Journal article
- Publication Details
- Genome research, Vol.14(10B), pp.2121-2127
- Publisher
- United States
- DOI
- 10.1101/gr.2596504
- PMID
- 15489334
- PMCID
- PMC528928
- ISSN
- 1088-9051
- eISSN
- 1549-5469
- Grant note
- N01-C0-12400 / PHS HHS
- Language
- English
- Date published
- 10/2004
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980053002771
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