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The structure of LsrB from Yersinia pestis complexed with autoinducer-2
Journal article   Open access   Peer reviewed

The structure of LsrB from Yersinia pestis complexed with autoinducer-2

Jeffrey S. Kavanaugh, Lokesh Gakhar and Alexander R. Horswill
Acta crystallographica. Section F, Structural biology communications, Vol.67(12), pp.1501-1505
12/2011
DOI: 10.1107/S1744309111042953
PMCID: PMC3232125
PMID: 22139152
url
https://europepmc.org/articles/pmc3232125View
Published (Version of record) Open Access

Abstract

The crystal structure of LsrB from Yersinia pestis complexed with autoinducer-2 (AI-2; space group P212121, unit-cell parameters a = 40.61, b = 61.03, c=125.23 angstrom) has been solved by molecular replacement using the structure of LsrB from Salmonella typhimurium (PDB entry ) and refined to R = 0.180 (Rfree = 0.213) at 1.75 angstrom resolution. The electron density for bound AI-2 and the stereochemistry of the AI-2-binding site are consistent with bound AI-2 adopting the (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran conformation, just as has been observed in the crystal structures of the Salmonella typhimurium and Sinorhizobium meliloti LsrBAI-2 complexes.
Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Crystallography Life Sciences & Biomedicine Physical Sciences Science & Technology

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