Journal article
The superficial tufted and mitral cell output neurons of the mouse olfactory bulb have dual roles in insulin sensing
Journal of cell science, Vol.138(19), jcs264088
10/01/2025
DOI: 10.1242/jcs.264088
PMCID: PMC12579955
PMID: 40969102
Abstract
The olfactory bulb (OB) contains multiple, parallel projection neurons to relay the nature of a stimulus. In a mouse ex vivo slice preparation, we used patch-clamp electrophysiology to measure intrinsic properties, excitability, action potential (AP) shape, voltage-activated conductances, and neuromodulation in the newly-categorized superficial tufted cells (sTCs) compared with those of mitral cells (MCs). We propose that a marked difference in voltage-dependent current represents distinct ion channel populations that affect the kinetics of action potentials, and evokes an increase in sTC firing frequency, albeit both types of projection neurons having similar AP spiking activity. Triple-colored immunofluorescence and RNA scope were used to detect co-localization of the Kv1.3 ion channel and the insulin receptor in sTCs, with ∼73% of sTCs expressing both. The sTCs were modulated by bath application of insulin - increasing AP firing frequency by 97%, attributable to an 8% decrease in the intraburst interval, and a reduction of the latency to first spike by 37%. We conclude that there may be a range of neuromodulators of sTCs that may alter excitability and fine-tune olfactory information processing or metabolic balance.
Details
- Title: Subtitle
- The superficial tufted and mitral cell output neurons of the mouse olfactory bulb have dual roles in insulin sensing
- Creators
- Louis John Kolling - Florida State UniversitySaptarsi Mitra - Florida State UniversityCatherine Anne Marcinkiewcz - Florida State UniversityDebra Ann Fadool - Florida State University
- Resource Type
- Journal article
- Publication Details
- Journal of cell science, Vol.138(19), jcs264088
- DOI
- 10.1242/jcs.264088
- PMID
- 40969102
- PMCID
- PMC12579955
- NLM abbreviation
- J Cell Sci
- ISSN
- 1477-9137
- eISSN
- 1477-9137
- Publisher
- COMPANY BIOLOGISTS LTD
- Grant note
- 2019-07151 / National Institute of Food and Agriculture DK133464 / NIDDK NIH HHS AG084196 / NIA NIH HHS HEERF II / Higher Education Emergency Relief Fund Robinson Family and Tallahasse Memorial Hospital
- Language
- English
- Electronic publication date
- 09/19/2025
- Date published
- 10/01/2025
- Academic Unit
- Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984963624102771
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