The tigA gene is a transcriptional fusion of glycolytic genes encoding triose-phosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase in oomycota

S E Unkles; J M Logsdon Jr; K Robison; J R Kinghorn; J M Duncan

doi:10.1128/jb.179.21.6816-6823.1997

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The tigA gene is a transcriptional fusion of glycolytic genes encoding triose-phosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase in oomycota

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The tigA gene is a transcriptional fusion of glycolytic genes encoding triose-phosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase in oomycota

S E Unkles, J M Logsdon Jr, K Robison, J R Kinghorn and J M Duncan

Journal of bacteriology, Vol.179(21), pp.6816-6823

11/1997

DOI: 10.1128/jb.179.21.6816-6823.1997

PMCID: PMC179613

PMID: 9352934

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Abstract

Genes encoding triose-phosphate isomerase (TPI) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are fused and form a single transcriptional unit (tigA) in Phytophthora species, members of the order Pythiales in the phylum Oomycota. This is the first demonstration of glycolytic gene fusion in eukaryotes and the first case of a TPI-GAPDH fusion in any organism. The tigA gene from Phytophthora infestans has a typical Oomycota transcriptional start point consensus sequence and, in common with most Phytophthora genes, has no introns. Furthermore, Southern and PCR analyses suggest that the same organization exists in other closely related genera, such as Pythium, from the same order (Oomycota), as well as more distantly related genera, Saprolegnia and Achlya, in the order Saprolegniales. Evidence is provided that in P. infestans, there is at least one other discrete copy of a GAPDH-encoding gene but not of a TPI-encoding gene. Finally, a phylogenetic analysis of TPI does not place Phytophthora within the assemblage of crown eukaryotes and suggests TPI may not be particularly useful for resolving relationships among major eukaryotic groups.

Amino Acid Sequence

Eukaryotic Cells

Evolution, Molecular

Genes, Fungal

Glyceraldehyde-3-Phosphate Dehydrogenases - genetics

Glycolysis

Molecular Sequence Data

Oomycetes - classification

Oomycetes - enzymology

Oomycetes - genetics

Phylogeny

Phytophthora - classification

Phytophthora - enzymology

Phytophthora - genetics

RNA, Fungal - genetics

RNA, Messenger - genetics

Sequence Homology, Amino Acid

Transcription, Genetic

Triose-Phosphate Isomerase - genetics

Details

Title: Subtitle: The tigA gene is a transcriptional fusion of glycolytic genes encoding triose-phosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase in oomycota
Creators: S E Unkles - Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom. shiela.unkles@med.monash.edu.au
J M Logsdon Jr
K Robison
J R Kinghorn
J M Duncan
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.179(21), pp.6816-6823
DOI: 10.1128/jb.179.21.6816-6823.1997
PMID: 9352934
PMCID: PMC179613
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Language: English
Date published: 11/1997
Academic Unit: Biology
Record Identifier: 9984217413602771

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