Journal article
The transmembrane nucleoporin Pom121 ensures efficient HIV-1 pre-integration complex nuclear import
Virology (New York, N.Y.), Vol.521, pp.169-174
08/2018
DOI: 10.1016/j.virol.2018.06.008
PMCID: PMC6309762
PMID: 29957337
Abstract
HIV-1 hijacks host classical cargo nuclear transportation, or nonclassical pathways by directly interacting with importin-β family proteins or nucleoporins for efficient pre-integration complex (PIC) nuclear import. Recently, an N-terminal truncated form of nucleoporin Pom121c (601–987 aa) was reported to inhibit HIV-1 replication. In contrast, we found that HIV-1 replication was significantly decreased in 293T and TZM-b1 cells with siRNA-mediated Pom121 knockdown. Quantitative PCR indicated that viral replication was impaired at the step of cDNA nuclear import. Furthermore, we found that karyopherin-β1 (KPNB1), which belongs to the importin-β family, interacts with Pom121 and is involved in Pom121-mediated PIC nuclear import. Rescue experiment indicated that the FG-repeats and the following α-helix in Pom121 are required for its role in HIV-1 PIC nuclear import. Taken together, our results showed that full-length Pom121 enables efficient PIC nuclear import, and suggested that this process may rely on KPNB1 dependent classical cargo nuclear transportation way.
•Pom121 promotes HIV-1 replication in vitro.•Pom121 facilitates HIV-1 PIC nuclear import.•FG-repeats domain in Pom121 is required for PIC nuclear import.•Pom121 mediated PIC nuclear import is KPNB1 dependent.
Details
- Title: Subtitle
- The transmembrane nucleoporin Pom121 ensures efficient HIV-1 pre-integration complex nuclear import
- Creators
- Jing Guo - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, ChinaXianxian Liu - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, ChinaChuanjian Wu - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, ChinaJingping Hu - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, ChinaKe Peng - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaLi Wu - Center for Retrovirus Research, Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USASidong Xiong - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, ChinaChunsheng Dong - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.521, pp.169-174
- DOI
- 10.1016/j.virol.2018.06.008
- PMID
- 29957337
- PMCID
- PMC6309762
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 81101257, 31470848, 31670898, 31470880; name: Open Research Fund Program of the State Key Laboratory of Virology of China, award: 2017IOV003; DOI: 10.13039/501100005065, name: Jiangsu Provincial Innovative Research Team; DOI: 10.13039/100000002, name: National Institutes of Health, award: AI104483, AI120209, GM128212
- Language
- English
- Date published
- 08/2018
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001123602771
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