The truncated C-terminal E2 (E2-TR) protein of bovine papillomavirus (BPV) type-1 is a transactivator that modulates transcription in vivo and in vitro in a manner distinct from the E2-TA and E8^E2 gene products

Michael J Lace; Masato Ushikai; Yasushi Yamakawa; James R Anson; Takaoki Ishiji; Lubomir P Turek; Thomas H Haugen

doi:10.1016/j.virol.2012.03.020

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The truncated C-terminal E2 (E2-TR) protein of bovine papillomavirus (BPV) type-1 is a transactivator that modulates transcription in vivo and in vitro in a manner distinct from the E2-TA and E8^E2 gene products

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The truncated C-terminal E2 (E2-TR) protein of bovine papillomavirus (BPV) type-1 is a transactivator that modulates transcription in vivo and in vitro in a manner distinct from the E2-TA and E8^E2 gene products

Michael J Lace, Masato Ushikai, Yasushi Yamakawa, James R Anson, Takaoki Ishiji, Lubomir P Turek and Thomas H Haugen

Virology (New York, N.Y.), Vol.429(2), pp.99-111

08/01/2012

DOI: 10.1016/j.virol.2012.03.020

PMID: 22551766

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Abstract

The E2 open reading frame of bovine papillomavirus (BPV)-1 encodes a 410 amino acid (aa) transcriptional activator, E2-TA, and collinear polypeptides—E2-TR (243 aa) and E8^E2 (196 aa). E8^E2 and E2-TR share the DNA-binding domain of E2-TA, and both have been defined as transcriptional repressors. Although purified E2-TR and E8^E2 proteins specifically bound E2 sites with similar affinities, only the E2-TR stimulated transcription. Here we show that E2-TR trans-activates E2-dependent promoters 5 to 10-fold in cooperation with cellular factors and in a dose-dependent fashion in epithelial cells and fibroblasts of animal or human origin while E2-TA activated >100-fold and the E8^E2 had no effect. However, in contrast to E2-TA, E2-TR activated transcription from a promoter-proximal position. E2-TR also partially inhibited the BPV-1 P89 or heterologous promoters whereas E8^E2 led to complete repression. Thus, the BPV-1 E2-TR modulates viral gene expression in a manner distinct from other E2 proteins.

Papillomavirus

Cooperative activation

Inhibition

Transcription

Gene expression

Details

Title: Subtitle: The truncated C-terminal E2 (E2-TR) protein of bovine papillomavirus (BPV) type-1 is a transactivator that modulates transcription in vivo and in vitro in a manner distinct from the E2-TA and E8^E2 gene products
Creators: Michael J Lace - Veterans Affairs Healthcare System, Iowa City, IA 52246, USA
Masato Ushikai - Veterans Affairs Healthcare System, Iowa City, IA 52246, USA
Yasushi Yamakawa - Veterans Affairs Healthcare System, Iowa City, IA 52246, USA
James R Anson - Veterans Affairs Healthcare System, Iowa City, IA 52246, USA
Takaoki Ishiji - Department of Dermatology, Jikei University School of Medicine, Tokyo, Japan
Lubomir P Turek - Veterans Affairs Healthcare System, Iowa City, IA 52246, USA
Thomas H Haugen - Veterans Affairs Healthcare System, Iowa City, IA 52246, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.429(2), pp.99-111
DOI: 10.1016/j.virol.2012.03.020
PMID: 22551766
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Grant note: name: merit awards to LPT and THH from the Department of Veterans Affairs, the NIH, award: CA-49912
Language: English
Date published: 08/01/2012
Academic Unit: Pathology
Record Identifier: 9984046806602771

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