The uptake of pristane (2,6,10,14-tetramethylpentadecane) into phospholipid bilayers as assessed by NMR, DSC, and tritium labeling methods

K Gawrisch; S Janz

doi:10.1016/0005-2736(91)90081-I

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The uptake of pristane (2,6,10,14-tetramethylpentadecane) into phospholipid bilayers as assessed by NMR, DSC, and tritium labeling methods

Journal article

Peer reviewed

The uptake of pristane (2,6,10,14-tetramethylpentadecane) into phospholipid bilayers as assessed by NMR, DSC, and tritium labeling methods

K Gawrisch and S Janz

Biochimica et biophysica acta, Vol.1070(2), pp.409-418

12/09/1991

DOI: 10.1016/0005-2736(91)90081-I

PMID: 1764453

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Abstract

Unilamellar dioleoylphosphatidylcholine (DOPC) liposomes (250 microM) incorporated 2 mol% of [3H]pristane at 37 degrees C after addition of 50 microM pristane solubilized with beta-cyclodextrin. Conventional solubilization in dimethyl sulphoxide resulted in much lower uptake. Premixing of perdeuterated pristane with DOPC and dipalmitoylphosphatidylcholine (DPPC) prior to the formation of multilamellar liposomes resulted in homogeneous incorporation of up to 5 mol% pristane at 22 degrees C and 50 degrees C, respectively, as observed by 2H-NMR. Lipid order parameters measured by 31P and 2H-NMR remained unchanged after pristane uptake. Pristane induced the transformation of part of the dioleoylphosphatidylethanolamine (DOPE)/DOPC (3:1, mol/mol) liquid crystalline lamellar phase into an inverse hexagonal phase. 5 mol% pristane in DPPC bilayers decreased the midpoint of the main phase transition temperature of DPPC from 41.5 degrees C to 40.9 degrees C. Upon cooling in the temperature range from 41 degrees C to 36 degrees C, pristane was either displaced from the DPPC bilayer or the mode of incorporation changed. These results may aid in defining the mechanisms whereby pristane, an isoprenoid C19-isoalkane, induces plasmacytomagenesis in mice.

Terpenes - chemistry

Magnetic Resonance Spectroscopy - methods

Lipid Bilayers

Models, Biological

Radioisotope Dilution Technique

Calorimetry, Differential Scanning - methods

Phospholipids - chemistry

Carcinogens - chemistry

Tritium

1,2-Dipalmitoylphosphatidylcholine - chemistry

Phosphatidylcholines - chemistry

Phosphatidylethanolamines - chemistry

Details

Title: Subtitle: The uptake of pristane (2,6,10,14-tetramethylpentadecane) into phospholipid bilayers as assessed by NMR, DSC, and tritium labeling methods
Creators: K Gawrisch - Division of Computer Research and Technology, National Institutes of Health, Bethesda, MD 20892
S Janz
Resource Type: Journal article
Publication Details: Biochimica et biophysica acta, Vol.1070(2), pp.409-418
DOI: 10.1016/0005-2736(91)90081-I
PMID: 1764453
ISSN: 0006-3002
eISSN: 1878-2434
Language: English
Date published: 12/09/1991
Academic Unit: Pathology
Record Identifier: 9984083293702771

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