The use of haptenated immunoglobulins to induce B cell tolerance in vitro: the roles of hapten density and the Fc portion of the immunoglobulin carrier

Thomas J. Waldschmidt; Yves Borel; Ellen S Vitetta

doi:10.4049/jimmunol.131.5.2204

Back

The use of haptenated immunoglobulins to induce B cell tolerance in vitro: the roles of hapten density and the Fc portion of the immunoglobulin carrier

Journal article

Peer reviewed

The use of haptenated immunoglobulins to induce B cell tolerance in vitro: the roles of hapten density and the Fc portion of the immunoglobulin carrier

Thomas J. Waldschmidt, Yves Borel and Ellen S Vitetta

The Journal of immunology (1950), Vol.131(5), pp.2204-2209

1983

DOI: 10.4049/jimmunol.131.5.2204

PMID: 6226736

View Online

Abstract

The relationship between the Fc region of trinitrophenylated (TNP)-immunoglobulins (Ig), and their ability to induce tolerance was examined. It was found that adult B cells responding to a T-independent (TI) antigen were tolerized by TNP11 human gamma globulin (HGG), but not by TNP10F(ab')2 fragments of HGG. Increasing the hapten density on the F(ab')2 fragments overcame their inability to induce tolerance. Thus, a TNP17-F(ab')2 was an effective tolerogen. Murine myeloma proteins of different IgG subclasses were similarly tested. A TNP12-IgG2a and a TNP11-IgG1 induced tolerance, whereas two TNP11-12-IgG3 did not. However, a more heavily haptenated TNP18-IgG3 was tolerogenic. These results suggest that lightly haptenated immunoglobulins depend upon Fc receptor binding to induce tolerance in adult B cells. Non-Fc receptor-binding carriers are not tolerogenic unless they are more heavily haptenated. Finally, T cell and macrophage depletion experiments suggest that the tolerogens act directly on the B cells.

Cell Interactions

Immunobiology

Fundamental and applied biological sciences. Psychology

Fundamental immunology

Biological and medical sciences

Analysis of the immune response. Humoral and cellular immunity

Details

Title: Subtitle: The use of haptenated immunoglobulins to induce B cell tolerance in vitro: the roles of hapten density and the Fc portion of the immunoglobulin carrier
Creators: Thomas J. Waldschmidt - Univ. Texas, health sci. center Dallas
Yves Borel - Univ. Texas, health sci. center Dallas
Ellen S Vitetta - Univ. Texas, health sci. center Dallas
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.131(5), pp.2204-2209
Publisher: American Association of Immunologists; Bethesda, MD
DOI: 10.4049/jimmunol.131.5.2204
PMID: 6226736
ISSN: 0022-1767
eISSN: 1550-6606
Language: English
Date published: 1983
Academic Unit: Pathology
Record Identifier: 9984046915702771

Metrics

22 Record Views

41 Times Cited - Web of Science