The use of saturated DCPD in remineralization of artificial caries lesions in vitro

J S Wefel; J D Harless

doi:10.1177/00220345870660110701

Back

The use of saturated DCPD in remineralization of artificial caries lesions in vitro

Journal article

Peer reviewed

The use of saturated DCPD in remineralization of artificial caries lesions in vitro

J S Wefel and J D Harless

Journal of dental research, Vol.66(11), pp.1640-1643

11/1987

DOI: 10.1177/00220345870660110701

PMID: 10872398

View Online

Abstract

Dicalcium phosphate dihydrate (DCPD) may play a significant role in the caries lesion since it is a stable calcium phosphate phase under acidic conditions. The reaction of DCPD and fluoride, forming fluorapatite (FAP), may provide a potentially promising treatment regimen for remineralization of caries lesions in vivo. The purpose of this study was to determine whether a two-step DCPD and inorganic wash with fluoride can remineralize artificial caries-like lesions in vitro. We used the single-section technique to facilitate quantitation of the same tissue before and after the experimental regimen. The two-step remineralizing treatment was repeated three times and consisted of a two-minute saturated DCPD treatment (pH 2.1) followed by a 24-hour inorganic wash. Lesion parameters were recorded before and after treatment by the taking of polarized light photomicrographs of each section after imbibition in several media. The changes in the tissue following treatment were expressed as a percent change in the area of the initial pre-treatment lesion. Significant reductions (p < 0.02) in lesion pore volume were observed in all aqueous media examined. In the lesions after imbibition in quinoline, remineralization was also apparent from the significant increase in the area of the dark zone following treatment. This two-step DCPD treatment appears to remineralize artificial caries-like lesions effectively, but additional work is needed to determine whether it affords any protection against subsequent cariogenic challenges.

Apatites - chemistry

Calcium Phosphates - administration & dosage

Calcium Phosphates - chemistry

Calcium Phosphates - therapeutic use

Cariostatic Agents - administration & dosage

Cariostatic Agents - chemistry

Cariostatic Agents - therapeutic use

Dental Caries - pathology

Dental Caries - therapy

Dental Enamel - drug effects

Dental Enamel - ultrastructure

Fluorides - administration & dosage

Fluorides - chemistry

Fluorides - therapeutic use

Follow-Up Studies

Humans

Hydrogen-Ion Concentration

Image Processing, Computer-Assisted

Microscopy, Polarization

Photomicrography

Porosity

Quinolines

Tooth Remineralization - methods

Water

Details

Title: Subtitle: The use of saturated DCPD in remineralization of artificial caries lesions in vitro
Creators: J S Wefel - University of Iowa
J D Harless - University of Iowa
Resource Type: Journal article
Publication Details: Journal of dental research, Vol.66(11), pp.1640-1643
DOI: 10.1177/00220345870660110701
PMID: 10872398
ISSN: 0022-0345
eISSN: 1544-0591
Grant note: DEO4486 / NIDCR NIH HHS
Language: English
Date published: 11/1987
Academic Unit: Dental Research
Record Identifier: 9984627330802771

Metrics

4 Record Views

31 Times Cited - Web of Science

See more details