Journal article
The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p
The EMBO journal, Vol.17(23), pp.6952-6962
12/01/1998
DOI: 10.1093/emboj/17.23.6952
PMCID: PMC1171043
PMID: 9843501
Abstract
The Saccharomyces cerevisiae Sln1 protein is a ‘two‐component’ regulator involved in osmotolerance. Two‐component regulators are a family of signal‐transduction molecules with histidine kinase activity common in prokaryotes and recently identified in eukaryotes. Phosphorylation of Sln1p inhibits the HOG1 MAP kinase osmosensing pathway via a phosphorelay mechanism including Ypd1p and the response regulator, Ssk1p. SLN1 also activates an MCM1‐dependent reporter gene, P‐lacZ, but this function is independent of Ssk1p. We present genetic and biochemical evidence that Skn7p is the response regulator for this alternative Sln1p signaling pathway. Thus, the yeast Sln1 phosphorelay is actually more complex than appreciated previously; the Sln1 kinase and Ypd1 phosphorelay intermediate regulate the activity of two distinct response regulators, Ssk1p and Skn7p. The established role of Skn7p in oxidative stress is independent of the conserved receiver domain aspartate, D427. In contrast, we show that Sln1p activation of Skn7p requires phosphorylation of D427. The expression of TRX2, previously shown to exhibit Skn7p‐dependent oxidative‐stress activation, is also regulated by the SLN1 phosphorelay functions of Skn7p. The identification of genes responsive to both classes of Skn7p function suggests a central role for Skn7p and the SLN1‐SKN7 pathway in integrating and coordinating cellular response to various types of environmental stress.
Details
- Title: Subtitle
- The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p
- Creators
- Sheng Li - Genetics PhD Program, University of IowaAddison Ault - Department of Biochemistry, University of IowaCheryl L Malone - Department of Biochemistry, University of IowaDesmond Raitt - Division of Yeast Genetics, National Institute for Medical ResearchSusan Dean - Department of Biological Sciences, University of IowaLeland H Johnston - Division of Yeast Genetics, National Institute for Medical ResearchRobert J Deschenes - Department of Biochemistry, University of IowaJan S Fassler - Department of Biological Sciences, University of Iowa
- Resource Type
- Journal article
- Publication Details
- The EMBO journal, Vol.17(23), pp.6952-6962
- Publisher
- John Wiley & Sons, Ltd
- DOI
- 10.1093/emboj/17.23.6952
- PMID
- 9843501
- PMCID
- PMC1171043
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- Number of pages
- 11
- Language
- English
- Date published
- 12/01/1998
- Academic Unit
- Biology
- Record Identifier
- 9984217429902771
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