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The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis
Journal article   Open access   Peer reviewed

The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis

M Schönbächler, A Horvath, J Fassler and H Riezman
The EMBO journal, Vol.14(8), pp.1637-1645
04/18/1995
DOI: 10.1002/j.1460-2075.1995.tb07152.x
PMCID: PMC398256
PMID: 7737116
url
https://doi.org/10.1002/j.1460-2075.1995.tb07152.xView
Published (Version of record) Open Access

Abstract

The protein encoded by the yeast gene SPT14 shows high sequence similarity to the human protein, PIG-A, whose loss of activity is at the origin of the disease paroxysmal nocturnal hemoglobinuria. The symptoms of this disease are apparently due to a loss of cell surface, glycosylphosphatidylinositol (GPI)-anchored proteins. Like PIG-A mutant cells, spt14 mutant cells are defective in GPI anchoring due to a defect in the synthesis of GlcNAc-PI, the first step of GPI synthesis. The spt14 mutant causes several other abnormalities including transcriptional defects and a downregulation of inositolphosphoceramide synthesis. We suggest that these defects are indirect results of the loss of GPI anchoring.

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