Journal article
The zinc indicator FluoZin-3 is not perturbed significantly by physiological levels of calcium or magnesium
Cell calcium (Edinburgh), Vol.44(4), pp.422-426
10/2008
DOI: 10.1016/j.ceca.2008.01.006
PMCID: PMC2572219
PMID: 18353435
Abstract
There has been some dispute in the literature as to the sensitivity of the zinc indicator FluoZin-3 to calcium, with suggestions that physiological levels of calcium and magnesium effectively occlude the response of the probe to zinc. In this communication we demonstrate that calcium concentrations as high as 10mM do not prevent FluoZin-3 from detecting zinc elevations as low as 100pM. Moreover, the inclusion of a few μM Ca-EDTA does not prevent FluoZin-3 from responding to increases in zinc concentration but does extend the dynamic range of the probe by reducing contaminating zinc levels and allowing the probe to respond to multiple zinc additions. In addition, we have derived a mathematical model to account for the kinetics of FluoZin-3 response to zinc in the presence of an additional zinc and calcium chelator.
Details
- Title: Subtitle
- The zinc indicator FluoZin-3 is not perturbed significantly by physiological levels of calcium or magnesium
- Creators
- Jinfu Zhao - Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USABryan A Bertoglio - Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USAKyle R GeeAlan R Kay - Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Cell calcium (Edinburgh), Vol.44(4), pp.422-426
- DOI
- 10.1016/j.ceca.2008.01.006
- PMID
- 18353435
- PMCID
- PMC2572219
- NLM abbreviation
- Cell Calcium
- ISSN
- 0143-4160
- eISSN
- 1532-1991
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 10/2008
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9983992099802771
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