The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis

Luka Sheppard; David G Green; Gerald Lerchbaumer; Katheryn E Rothenberg; Rodrigo Fernandez-Gonzalez; Ulrich Tepass

doi:10.1083/jcb.202108091

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The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis

Journal article

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The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis

Luka Sheppard, David G Green, Gerald Lerchbaumer, Katheryn E Rothenberg, Rodrigo Fernandez-Gonzalez and Ulrich Tepass

The Journal of cell biology, Vol.222(2), p.1

02/06/2023

DOI: 10.1083/jcb.202108091

PMCID: PMC9757846

PMID: 36520419

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Abstract

α-Catenin couples the cadherin-catenin complex to the actin cytoskeleton. The mechanosensitive α-Catenin M region undergoes conformational changes upon application of force to recruit interaction partners. Here, we took advantage of the tension landscape in the Drosophila embryo to define three different states of α-Catenin mechanosensing in support of cell adhesion. Low-, medium-, and high-tension contacts showed a corresponding recruitment of Vinculin and Ajuba, which was dependent on the α-Catenin M region. In contrast, the Afadin homolog Canoe acts in parallel to α-Catenin at bicellular low- and medium-tension junctions but requires an interaction with α-Catenin for its tension-sensitive enrichment at high-tension tricellular junctions. Individual M region domains make complex contributions to cell adhesion through their impact on interaction partner recruitment, and redundancies with the function of Canoe. Our data argue that α-Catenin and its interaction partners are part of a cooperative and partially redundant mechanoresponsive network that supports AJs remodeling during morphogenesis.

Actin Cytoskeleton - metabolism

Actins - metabolism

Adherens Junctions - metabolism

alpha Catenin - genetics

alpha Catenin - metabolism

Animals

Cadherins - genetics

Cadherins - metabolism

Cell Adhesion

Drosophila

Drosophila Proteins - genetics

Drosophila Proteins - metabolism

Morphogenesis

Vinculin - genetics

Vinculin - metabolism

Details

Title: Subtitle: The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
Creators: Luka Sheppard - University of Toronto
David G Green - University of Toronto
Gerald Lerchbaumer - University of Toronto
Katheryn E Rothenberg - University of Toronto
Rodrigo Fernandez-Gonzalez - Developmental and Stem Cell Biology Program, The Hospital for Sick Children, Toronto, Canada
Ulrich Tepass - University of Toronto
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.222(2), p.1
DOI: 10.1083/jcb.202108091
PMID: 36520419
PMCID: PMC9757846
NLM abbreviation: J Cell Biol
ISSN: 0021-9525
eISSN: 1540-8140
Grant note: CIHR
Language: English
Date published: 02/06/2023
Academic Unit: Biology
Record Identifier: 9984696568002771

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