Journal article
Theoretical Considerations for Optimizing the Use of Optogenetics with Complex Behavior
Current protocols, Vol.3(7), e836
07/01/2023
DOI: 10.1002/cpz1.836
PMCID: PMC10406170
PMID: 37439512
Appears in UI Libraries Support Open Access
Abstract
Optogenetic approaches have allowed researchers to address complex questions about behavior that were previously unanswerable. However, as optogenetic procedures involve a large parameter space across multiple dimensions, it is crucial to consider such parameters in conjunction with the behaviors under study. Here, we discuss strategies to optimize optogenetic approaches with complex behavior by identifying critical experimental design considerations, including frequency specificity, temporal precision, activity-controlled optogenetics, stimulation pattern, and cell-type specificity. We highlight potential limitations or theoretical considerations to be made when manipulating each of these factors of optogenetic experiments. This overview emphasizes the importance of optimizing optogenetic study design to enhance the conclusions that can be drawn about the neuroscience of behavior.
Details
- Title: Subtitle
- Theoretical Considerations for Optimizing the Use of Optogenetics with Complex Behavior
- Creators
- Bess Glickman - University of IowaRyan T LaLumiere - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Current protocols, Vol.3(7), e836
- DOI
- 10.1002/cpz1.836
- PMID
- 37439512
- PMCID
- PMC10406170
- NLM abbreviation
- Curr Protoc
- eISSN
- 2691-1299
- Publisher
- Wiley
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: MH132207, DA049139, DA048055
- Language
- English
- Date published
- 07/01/2023
- Academic Unit
- Psychological and Brain Sciences; Iowa Neuroscience Institute
- Record Identifier
- 9984444765602771
Metrics
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