There and back again: new single-molecule insights in the motion of DNA repair proteins

Maria Spies

doi:10.1016/j.sbi.2012.11.008

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Journal article

Peer reviewed

There and back again: new single-molecule insights in the motion of DNA repair proteins

Maria Spies

Current opinion in structural biology, Vol.23(1), pp.154-160

02/2013

DOI: 10.1016/j.sbi.2012.11.008

PMID: 23260129

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Abstract

Cellular DNA repair machines are constantly at work supporting the integrity of our genomes. Numerous proteins cooperate to form a complex and adaptive system dedicated to detection and timely processing of DNA damage. The molecular underpinnings of how these proteins locate and discriminate DNA lesions, match homologous sequences, mend the DNA and attend to a replication in distress are of a paramount biomedical importance, but in many cases remain unclear. Combined with more conventional tools, single-molecule biochemistry has been stepping in to address the age-old problems in the DNA repair field. This review will address new insights into diffusive properties of three DNA repair systems: I will discuss the emerging model of how MutS homologues locate and respond to mismatches in the dsDNA; the mechanism by which RAD52 promotes annealing of complementary DNA strands coated with ssDNA binding protein RPA; and how the nucleoprotein filament formed by RecA recombinase on ssDNA searches for homology within duplex DNA. These three distinct DNA repair factors exemplify the dynamic nature of cellular DNA repair machines revealed by single-molecule studies.

DNA, Single-Stranded - genetics

DNA-Binding Proteins - metabolism

Rad52 DNA Repair and Recombination Protein - chemistry

Rec A Recombinases - metabolism

Rad52 DNA Repair and Recombination Protein - metabolism

DNA Repair

Protein Binding

Rec A Recombinases - chemistry

DNA-Binding Proteins - chemistry

DNA, Single-Stranded - chemistry

Details

Title: Subtitle: There and back again: new single-molecule insights in the motion of DNA repair proteins
Creators: Maria Spies - Department of Biochemistry, University of Iowa, Iowa City, IA, USA. maria-spies@uiowa.edu
Resource Type: Journal article
Publication Details: Current opinion in structural biology, Vol.23(1), pp.154-160
Publisher: England
DOI: 10.1016/j.sbi.2012.11.008
PMID: 23260129
ISSN: 0959-440X
eISSN: 1879-033X
Grant note: Howard Hughes Medical Institute
Language: English
Date published: 02/2013
Academic Unit: Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984024521502771

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