Journal article
Thermal mitigation of Pseudomonas aeruginosa biofilms
Biofouling (Chur, Switzerland), Vol.31(8), pp.665-675
09/14/2015
DOI: 10.1080/08927014.2015.1083985
PMCID: PMC4617618
PMID: 26371591
Abstract
Bacterial biofilms infect 2-4% of medical devices upon implantation, resulting in multiple surgeries and increased recovery time due to the very great increase in antibiotic resistance in the biofilm phenotype. This work investigates the feasibility of thermal mitigation of biofilms at physiologically accessible temperatures. Pseudomonas aeruginosa biofilms were cultured to high bacterial density (1.7 × 10
9
CFU cm
−2
) and subjected to thermal shocks ranging from 50°C to 80°C for durations of 1-30 min. The decrease in viable bacteria was closely correlated with an Arrhenius temperature dependence and Weibull-style time dependence, demonstrating up to six orders of magnitude reduction in bacterial load. The bacterial load for films with more conventional initial bacterial densities dropped below quantifiable levels, indicating thermal mitigation as a viable approach to biofilm control.
Details
- Title: Subtitle
- Thermal mitigation of Pseudomonas aeruginosa biofilms
- Creators
- Ann O'Toole - Department of Chemical and Biochemical Engineering, 4133 Seamans Center for the Engineering Arts & Sciences, University of IowaErica B Ricker - Department of Chemical and Biochemical Engineering, 4133 Seamans Center for the Engineering Arts & Sciences, University of IowaEric Nuxoll - Department of Chemical and Biochemical Engineering, 4133 Seamans Center for the Engineering Arts & Sciences, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Biofouling (Chur, Switzerland), Vol.31(8), pp.665-675
- DOI
- 10.1080/08927014.2015.1083985
- PMID
- 26371591
- PMCID
- PMC4617618
- NLM abbreviation
- Biofouling
- ISSN
- 0892-7014
- eISSN
- 1029-2454
- Publisher
- Taylor & Francis
- Grant note
- CBET-1133297 / Division of Chemical, Bioengineering, Environmental, and Transport Systems (10.13039/100000146) 11SDG7600044 / American Heart Association (10.13039/100000968) 5T32GM008365 / National Institute of General Medical Sciences (10.13039/100000057)
- Language
- English
- Date published
- 09/14/2015
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Chemical and Biochemical Engineering
- Record Identifier
- 9984003949402771
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