Journal article
Thiamine Deficiency Induces Anorexia by Inhibiting Hypothalamic AMPK
Neuroscience, Vol.267, pp.102-113
05/16/2014
DOI: 10.1016/j.neuroscience.2014.02.033
PMCID: PMC3995829
PMID: 24607345
Abstract
Obesity and eating disorders are prevailing health concerns worldwide. It is important to understand the regulation of food intake and energy metabolism. Thiamine (vitamin B1) is an essential nutrient. Thiamine deficiency (TD) can cause a number of disorders in humans, such as Beriberi and Wernicke-Korsakoff syndrome. We demonstrated here that TD caused anorexia in C57BL/6 mice. After feeding a TD diet for 16 days, the mice displayed a significant decrease in food intake and an increase in resting energy expenditure (REE), which resulted in a severe weight loss. At the 22
nd
day, the food intake was reduced by 69% and 74% for male and female mice, respectively in TD group. The REE increased by 9 folds in TD group. The loss of body weight (17–24%) was similar between male and female animals and mainly resulted from the reduction of fat mass (49% decrease). Re-supplementation of thiamine (benfotiamine) restored animal's appetite, leading to a total recovery of body weight. The hypothalamic AMPK is a critical regulator of food intake. TD inhibited the phosphorylation of AMPK in the arcuate nucleus (ARN) and paraventricular nucleus (PVN) of the hypothalamus without affecting its expression. TD-induced inhibition of AMPK phosphorylation was reversed once thiamine was re-supplemented. In contrast, TD increased AMPK phosphorylation in the skeletal muscle and upregulated the uncoupling protein (UCP)-1 in brown adipose tissues which was consistent with increased basal energy expenditure. Re-administration of thiamine stabilized AMPK phosphorylation in the skeletal muscle as well as energy expenditure. Taken together, TD may induce anorexia by inhibiting hypothalamic AMPK activity. With a simultaneous increase in energy expenditure, TD caused an overall body weight loss. The results suggest that the status of thiamine levels in the body may affect food intake and body weight.
Details
- Title: Subtitle
- Thiamine Deficiency Induces Anorexia by Inhibiting Hypothalamic AMPK
- Creators
- Mei Liu - University of KentuckyAlexander Alimov - University of KentuckyHaiping Wang - University of KentuckyJacqueline A Frank - University of KentuckyWendy Katz - University of KentuckyMei Xu - University of KentuckyZun-Ji Ke - Shanghai Institutes for Biological SciencesJia Luo - University of Kentucky
- Resource Type
- Journal article
- Publication Details
- Neuroscience, Vol.267, pp.102-113
- DOI
- 10.1016/j.neuroscience.2014.02.033
- PMID
- 24607345
- PMCID
- PMC3995829
- NLM abbreviation
- Neuroscience
- ISSN
- 0306-4522
- eISSN
- 1873-7544
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health (NIH), award: AA015407-09
- Language
- English
- Date published
- 05/16/2014
- Academic Unit
- Pathology
- Record Identifier
- 9984201118702771
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