Journal article
Thiamine deficiency promotes T cell infiltration in experimental autoimmune encephalomyelitis: the involvement of CCL2
The Journal of immunology (1950), Vol.193(5), pp.2157-2167
09/01/2014
DOI: 10.4049/jimmunol.1302702
PMCID: PMC4135011
PMID: 25063874
Abstract
Multiple sclerosis (MS) is a complex multifactorial disease that results from the interplay between environmental factors and a susceptible genetic background. Experimental autoimmune encephalomyelitis (EAE) has been widely used to investigate the mechanisms underlying MS pathogenesis. Chemokines, such as CCL2, are involved in the development of EAE. We have previously shown that thiamine deficiency (TD) induced CCL2 in neurons. We hypothesized that TD may affect the pathogenesis of EAE. In this study, EAE was induced in C57BL/6J mice by the injection of myelin oligodendroglial glycoprotein (MOG) peptides 35-55 with or without TD. TD aggravated the development of EAE, which was indicated by clinical scores and pathologic alterations in the spinal cord. TD also accelerated the development of EAE in an adoptive transfer EAE model. TD caused microglial activation and a drastic increase (up 140%) in leukocyte infiltration in the spinal cord of the EAE mice; specifically, TD increased Th1 and Th17 cells. TD upregulated the expression of CCL2 and its receptor CCR2 in the spinal cord of EAE mice. Cells in peripheral lymph node and spleen isolated from MOG-primed TD mice showed much stronger proliferative responses to MOG. CCL2 stimulated the proliferation and migration of T lymphocytes in vitro. Our results suggested that TD exacerbated the development of EAE through activating CCL2 and inducing pathologic inflammation.
Details
- Title: Subtitle
- Thiamine deficiency promotes T cell infiltration in experimental autoimmune encephalomyelitis: the involvement of CCL2
- Creators
- Zhe Ji - Chinese Academy of SciencesZhiqin Fan - Chinese Academy of SciencesYing Zhang - Chinese Academy of SciencesRonghuan Yu - Chinese Academy of SciencesHaihua Yang - Chinese Academy of SciencesChenghua Zhou - Chinese Academy of SciencesJia Luo - University of KentuckyZun-Ji Ke - Shanghai University of Traditional Chinese Medicine
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.193(5), pp.2157-2167
- DOI
- 10.4049/jimmunol.1302702
- PMID
- 25063874
- PMCID
- PMC4135011
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AA015407 / NIAAA NIH HHS R01 AA015407 / NIAAA NIH HHS R21 AA019693 / NIAAA NIH HHS AA019693 / NIAAA NIH HHS
- Language
- English
- Date published
- 09/01/2014
- Academic Unit
- Pathology
- Record Identifier
- 9984201257102771
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