Three-dimensional bioprinting using self-assembling scalable scaffold-free "tissue strands" as a new bioink

Yin Yu; Kazim K Moncal; Jianqiang Li; Weijie Peng; Iris Rivero; James A Martin; Ibrahim T Ozbolat

doi:10.1038/srep28714

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Three-dimensional bioprinting using self-assembling scalable scaffold-free "tissue strands" as a new bioink

Journal article

Open access

Peer reviewed

Three-dimensional bioprinting using self-assembling scalable scaffold-free "tissue strands" as a new bioink

Yin Yu, Kazim K Moncal, Jianqiang Li, Weijie Peng, Iris Rivero, James A Martin and Ibrahim T Ozbolat

Scientific reports, Vol.6(1), pp.28714-28714

06/27/2016

DOI: 10.1038/srep28714

PMCID: PMC4921918

PMID: 27346373

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Abstract

Recent advances in bioprinting have granted tissue engineers the ability to assemble biomaterials, cells, and signaling molecules into anatomically relevant functional tissues or organ parts. Scaffold-free fabrication has recently attracted a great deal of interest due to the ability to recapitulate tissue biology by using self-assembly, which mimics the embryonic development process. Despite several attempts, bioprinting of scale-up tissues at clinically-relevant dimensions with closely recapitulated tissue biology and functionality is still a major roadblock. Here, we fabricate and engineer scaffold-free scalable tissue strands as a novel bioink material for robotic-assisted bioprinting technologies. Compare to 400 μm-thick tissue spheroids bioprinted in a liquid delivery medium into confining molds, near 8 cm-long tissue strands with rapid fusion and self-assemble capabilities are bioprinted in solid form for the first time without any need for a scaffold or a mold support or a liquid delivery medium, and facilitated native-like scale-up tissues. The prominent approach has been verified using cartilage strands as building units to bioprint articular cartilage tissue.

Materials Testing

Tissue Engineering

Animals

Cartilage, Articular - cytology

Cartilage, Articular - metabolism

Cattle

Printing, Three-Dimensional

Tissue Scaffolds - chemistry

Details

Title: Subtitle: Three-dimensional bioprinting using self-assembling scalable scaffold-free "tissue strands" as a new bioink
Creators: Yin Yu - The Center for Engineering in Medicine, Massachusetts General Hospital, Boston, MA, USA
Kazim K Moncal - Huck Institute of Life Sciences, The Pennsylvania State University, State College, PA, USA
Jianqiang Li - Industrial and Manufacturing Systems Engineering, Iowa State University, Ames, IA, USA
Weijie Peng - Engineering Science and Mechanics Department, The Pennsylvania State University, State College, PA, USA
Iris Rivero - Industrial and Manufacturing Systems Engineering, Iowa State University, Ames, IA, USA
James A Martin - Department of Orthopaedics and Rehabilitation, The University of Iowa, Iowa City, IA, USA
Ibrahim T Ozbolat - Huck Institute of Life Sciences, The Pennsylvania State University, State College, PA, USA
Resource Type: Journal article
Publication Details: Scientific reports, Vol.6(1), pp.28714-28714
DOI: 10.1038/srep28714
PMID: 27346373
PMCID: PMC4921918
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Publisher: England
Language: English
Date published: 06/27/2016
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation
Record Identifier: 9984040009502771

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