Three-dimensional culture environments enhance osteoblast differentiation

Jessica Boehrs; Rebecca S Zaharias; John Laffoon; Y Joon Ko; Galen B Schneider

doi:10.1111/j.1532-849X.2008.00330.x

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Three-dimensional culture environments enhance osteoblast differentiation

Journal article

Open access

Peer reviewed

Three-dimensional culture environments enhance osteoblast differentiation

Jessica Boehrs, Rebecca S Zaharias, John Laffoon, Y Joon Ko and Galen B Schneider

Journal of prosthodontics, Vol.17(7), pp.517-521

10/2008

DOI: 10.1111/j.1532-849X.2008.00330.x

PMID: 18573152

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https://doi.org/10.1111/j.1532-849X.2008.00330.xView

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Abstract

In previous work from our laboratory, we demonstrated that the three-dimensional (3D) cell cultures developed in simulated microgravity environments enhanced osseous-like aggregate formation and accelerated preosteoblast cell differentiation. Thus, as described here, we hypothesize that aggregate formation and mineralization would occur with fewer than 10 x 10(6) cells as previously described. Human preosteoblastic cells were cultured at different concentrations in a rotary wall vessel to simulate microgravity for 7 days. Aggregate size was assessed, and mineralization and collagen expression detected using Von Kossa and Masson Trichrome staining. Scanning electron microscopy was used for structural and elemental analysis. Immunohistochemistry was used to detect expression of the osteogenic markers BSPII and osteopontin (OP). Size and calcium expression were dependent upon cultured cell number (p < 0.01). Calcium and collagen expression were detected throughout the aggregate, but organization was independent of cell number. Aggregates had similar microscopic structural patterns demonstrating organized development. Presence of BSPII and OP showed that the aggregates share common differentiation proteins with in vivo bone formation. These results may lead to novel bone engineering techniques associated with dental treatment.

Immunohistochemistry

Integrin-Binding Sialoprotein

Cell Count

Humans

Cells, Cultured

Electron Probe Microanalysis

Palate, Hard - embryology

Weightlessness

Sialoglycoproteins - biosynthesis

Mesenchymal Stromal Cells - cytology

Calcification, Physiologic

Osteopontin - biosynthesis

Cell Differentiation

Collagen - biosynthesis

Cell Culture Techniques

Osteoblasts - cytology

Osteoblasts - metabolism

Details

Title: Subtitle: Three-dimensional culture environments enhance osteoblast differentiation
Creators: Jessica Boehrs - University of Iowa College of Dentistry, Iowa City, IA 52242-1001, USA
Rebecca S Zaharias
John Laffoon
Y Joon Ko
Galen B Schneider
Resource Type: Journal article
Publication Details: Journal of prosthodontics, Vol.17(7), pp.517-521
DOI: 10.1111/j.1532-849X.2008.00330.x
PMID: 18573152
NLM abbreviation: J Prosthodont
ISSN: 1059-941X
eISSN: 1532-849X
Publisher: United States
Grant note: R21-DE016677-01 / NIDCR NIH HHS
Language: English
Date published: 10/2008
Academic Unit: Dentistry Administration; Prosthodontics
Record Identifier: 9984065805102771

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