Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation

Qingqing Yao; Jaqueline G L Cosme; Tao Xu; Jacob M Miszuk; Paulo H S Picciani; Hao Fong; Hongli Sun

doi:10.1016/j.biomaterials.2016.11.018

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Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation

Journal article

Open access

Peer reviewed

Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation

Qingqing Yao, Jaqueline G L Cosme, Tao Xu, Jacob M Miszuk, Paulo H S Picciani, Hao Fong and Hongli Sun

Biomaterials, Vol.115, pp.115-127

01/2017

DOI: 10.1016/j.biomaterials.2016.11.018

PMCID: PMC5181114

PMID: 27886552

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Abstract

Nanofibrous scaffolds that are morphologically/structurally similar to natural ECM are highly interested for tissue engineering; however, the electrospinning technique has the difficulty in directly producing clinically relevant 3D nanofibrous scaffolds with desired structural properties. To address this challenge, we have developed an innovative technique of thermally induced nanofiber self-agglomeration (TISA) recently. The aim of this work was to prepare (via the TISA technique) and evaluate 3D electrospun PCL/PLA blend (mass ratio: 4/1) nanofibrous scaffolds having high porosity of ∼95.8% as well as interconnected and hierarchically structured pores with sizes from sub-micrometers to ∼300 μm for bone tissue engineering. The hypothesis was that the incorporation of PLA (with higher mechanical stiffness/modulus and bioactivity) into PCL nanofibers would significantly improve human mesenchymal stem cells (hMSCs) osteogenic differentiation in vitro and bone formation in vivo. Compared to neat PCL-3D scaffolds, PCL/PLA-3D blend scaffolds had higher mechanical properties and in vitro bioactivity; as a result, they not only enhanced the cell viability of hMSCs but also promoted the osteogenic differentiation. Furthermore, our in vivo studies revealed that PCL/PLA-3D scaffolds considerably facilitated new bone formation in a critical-sized cranial bone defect mouse model. In summary, both in vitro and in vivo results indicated that novel 3D electrospun PCL/PLA blend nanofibrous scaffolds would be strongly favorable/desired for hMSCs osteogenic differentiation and cranial bone formation.

Cell Differentiation

Materials Testing

Animals

Electroplating - methods

Equipment Design

Male

Mice

Mice, Inbred C57BL

Mice, Inbred Strains

Nanofibers - chemistry

Osteogenesis - physiology

Polyesters - chemistry

Printing, Three-Dimensional

Rotation

Skull Fractures - pathology

Skull Fractures - physiopathology

Skull Fractures - therapy

Stem Cell Transplantation - instrumentation

Stem Cell Transplantation - methods

Stem Cells - cytology

Stem Cells - physiology

Tissue Engineering - instrumentation

Tissue Engineering - methods

Tissue Scaffolds

Treatment Outcome

Details

Title: Subtitle: Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation
Creators: Qingqing Yao - University of South Dakota
Jaqueline G L Cosme - South Dakota School of Mines and Technology
Tao Xu - South Dakota School of Mines and Technology
Jacob M Miszuk - University of South Dakota
Paulo H S Picciani - Universidade Federal do Rio de Janeiro
Hao Fong - South Dakota School of Mines and Technology
Hongli Sun - University of South Dakota
Resource Type: Journal article
Publication Details: Biomaterials, Vol.115, pp.115-127
DOI: 10.1016/j.biomaterials.2016.11.018
PMID: 27886552
PMCID: PMC5181114
NLM abbreviation: Biomaterials
ISSN: 0142-9612
eISSN: 1878-5905
Grant note: P20 GM103548 / NIGMS NIH HHS P20 GM103620 / NIGMS NIH HHS
Language: English
Date published: 01/2017
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
Record Identifier: 9984367628502771

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