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Thyroid hormone controls multiple independent programs required for limb development in Xenopus laevis metamorphosis
Journal article   Open access   Peer reviewed

Thyroid hormone controls multiple independent programs required for limb development in Xenopus laevis metamorphosis

Donald D. Brown, Liquan Cai, Biswajit Das, Nicholas Marsh-Armstrong, Alexander M. Schreiber and Rejeanne Juste
Proceedings of the National Academy of Sciences - PNAS, Vol.102(35), pp.12455-12458
08/29/2005
DOI: 10.1073/pnas.0505989102
PMCID: PMC1194953
PMID: 16129821
url
https://doi.org/10.1073/pnas.0505989102View
Published (Version of record) Open Access

Abstract

Thyroid hormone (TH) is required for limb development in Xenopus laevis . Specific cell types in the growing limb were targeted for expression of a dominant negative form of the TH receptor by sperm-mediated transgenesis. Limb muscle development, the innervation of muscle from the spinal cord, and cartilage growth can be inhibited without affecting patterning of the limb or differentiation of other cell types. Remodeling of the skin occurs late in metamorphosis after the limb has formed. The coordination of these independent programs is affected in part by the control that TH exerts over DNA replication in all cell types of the limb.
Biological Sciences cell autonomy DNA replication thyroid hormone receptor transgenesis

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