Journal article
Time- and dose-dependent differential regulation of copper-zinc superoxide dismutase and manganese superoxide dismutase enzymatic activity and mRNA level by vitamin E in rat blood cells
Redox report : communications in free radical research, Vol.17(3), pp.101-107
05/01/2012
DOI: 10.1179/1351000212Y.0000000008
PMCID: PMC6837400
PMID: 22732938
Abstract
Background
Vitamin E is the most important lipid-soluble antioxidant. Recently, it has been proposed as a gene regulator, and its gene modulation effects have been observed at different levels of gene expression and cell signaling. This study was performed to investigate the effects of vitamin E on the activity and expression of the most important endogenous antioxidant enzyme, superoxide dismutase (SOD), in rat plasma.
Methods
Twenty-eight male Sprauge-Dawley rats were divided into four groups: control group and three dosing groups. The control group received the vehicle (liquid paraffin), and the dosing groups received twice-weekly intraperitoneal injections of 10, 30, and 100 mg/kg of vitamin E ((±)-α-Tocopherol) for 6 weeks. Quantitative real-time reverse transcription-polymerase chain reaction and enzyme assays were used to assess the levels of Cu/Zn-SOD and Mn-SOD mRNA and enzyme activity levels in blood cells at 0, 2, 4, and 6 weeks following vitamin E administration. Catalase enzyme activity and total antioxidant capacity were also assessed in plasma at the same time intervals.
Results
Mn-SOD activity was significantly increased in the 100 and 30 mg/kg dosing groups after 4 and 6 weeks, with corresponding significant increase in their mRNA levels. Cu/Zn-SOD activity was not significantly changed in response to vitamin E administration at any time points, whereas Cu/Zn-SOD mRNA levels were significantly increased after longer time points with high doses (30 and 100 mg/kg) of vitamin E. Catalase enzyme activity was transiently but significantly increased after 4 weeks of vitamin E treatment in 30 and 100 mg/kg dosing groups. Total antioxidant status was significantly increased after 4 and 6 weeks in the 100 mg/kg dosing group.
Conclusion
Only the chronic administration of higher doses of alpha-tocopherol is associated with the increased activity and expression of Mn-SOD in rats. Cu/Zn-SOD activity and expression does not dramatically change in response to vitamin E.
Details
- Title: Subtitle
- Time- and dose-dependent differential regulation of copper-zinc superoxide dismutase and manganese superoxide dismutase enzymatic activity and mRNA level by vitamin E in rat blood cells
- Creators
- Maliheh Hajiani - Tehran University of Medical SciencesFarideh Razi - Tehran University of Medical SciencesAboualfazl Golestani - Department of BiochemistryTehran University of Medical Sciences, Tehran, IranMehdi Frouzandeh - Tarbiat Modares UniversityAli Akbar Owji - Shiraz University of Medical SciencesShahnaz Khaghani - Department of BiochemistryTehran University of Medical Sciences, Tehran, IranNaghmeh Ghannadian - Tehran University of Medical SciencesAhmad Shariftabrizi - Department of BiochemistryTehran University of Medical Sciences, Tehran, IranParvin Pasalar - Department of BiochemistryTehran University of Medical Sciences, Tehran, Iran; Center for Research on Occupational Diseases (GRAD)Tehran, Iran
- Resource Type
- Journal article
- Publication Details
- Redox report : communications in free radical research, Vol.17(3), pp.101-107
- Publisher
- Taylor & Francis
- DOI
- 10.1179/1351000212Y.0000000008
- PMID
- 22732938
- PMCID
- PMC6837400
- ISSN
- 1351-0002
- eISSN
- 1743-2928
- Language
- English
- Date published
- 05/01/2012
- Academic Unit
- Radiology
- Record Identifier
- 9984318691802771
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