Journal article
Time course of active and passive liquid and solute movement in the isolated perfused rat lung model
Journal of applied physiology (1985), Vol.85(4), pp.1572-1577
10/01/1998
DOI: 10.1152/jappl.1998.85.4.1572
PMID: 9760355
Abstract
The isolated perfused rat lung model (IL) is used to study alveolar epithelial transport properties. Most of the previous studies have been done over a short period of time and have not used the same preparation as a control and intervention group. We evaluated whether the IL preparation could be used for a prolonged period of time (5 h) and studied the rates of active Na
+
transport, lung liquid clearance, and passive movement of solutes. Active Na
+
transport and lung liquid clearance were stable from 1 to 5 h. The passive movement of small solutes (Na
+
, mannitol) did not change significantly, and albumin movement increased slightly at the fifth hour. Total RNA isolated from IL after 5 h was intact, and the Na
+
-K
+
-ATPase activity in alveolar type II cells isolated at the end of 5-h experiments was equal to Na
+
-K
+
-ATPase function from freshly isolated alveolar type II cells. Finally, we measured the stimulatory effect of the β-adrenergic-agonist terbutaline and the inhibitory effect of the Na
+
-K
+
-ATPase-antagonist ouabain by using the same animal as a control. Accordingly, the isolated perfused lung model is functionally stable for at least 5 h, and it could be utilized to evaluate the effect of different interventions by using the same preparation.
Details
- Title: Subtitle
- Time course of active and passive liquid and solute movement in the isolated perfused rat lung model
- Creators
- F. J. Saldías - Pontificia Universidad Católica de ChileA. Comellas - University of Illinois at ChicagoC. Guerrero - University of Illinois at ChicagoK. M. Ridge - University of Illinois at ChicagoD. H. Rutschman - University of Illinois at ChicagoJ. I. Sznajder - University of Illinois at Chicago
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.85(4), pp.1572-1577
- DOI
- 10.1152/jappl.1998.85.4.1572
- PMID
- 9760355
- ISSN
- 8750-7587
- eISSN
- 1522-1601
- Language
- English
- Date published
- 10/01/1998
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; ICTS; Internal Medicine
- Record Identifier
- 9984359823602771
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