Journal article
Time course of pulmonary inflammation and trace element biodistribution during and after sub-acute inhalation exposure to copper oxide nanoparticles in a murine model
Particle and fibre toxicology, Vol.19(1), pp.40-40
06/13/2022
DOI: 10.1186/s12989-022-00480-z
PMCID: PMC9195454
PMID: 35698146
Abstract
BACKGROUNDIt has been shown that copper oxide nanoparticles (CuO NPs) induce pulmonary toxicity after acute or sub-acute inhalation exposures. However, little is known about the biodistribution and elimination kinetics of inhaled CuO NPs from the respiratory tract. The purposes of this study were to observe the kinetics of pulmonary inflammation during and after CuO NP sub-acute inhalation exposure and to investigate copper (Cu) biodistribution and clearance rate from the exposure site and homeostasis of selected trace elements in secondary organs of BALB/c mice. RESULTSSub-acute inhalation exposure to CuO NPs led to pulmonary inflammation represented by increases in lactate dehydrogenase, total cell counts, neutrophils, macrophages, inflammatory cytokines, iron levels in bronchoalveolar lavage (BAL) fluid, and lung weight changes. Dosimetry analysis in lung tissues and BAL fluid showed Cu concentration increased steadily during exposure and gradually declined after exposure. Cu elimination from the lung showed first-order kinetics with a half-life of 6.5 days. Total Cu levels were significantly increased in whole blood and heart indicating that inhaled Cu could be translocated into the bloodstream and heart tissue, and potentially have adverse effects on the kidneys and spleen as there were significant changes in the weights of these organs; increase in the kidneys and decrease in the spleen. Furthermore, concentrations of selenium in kidneys and iron in spleen were decreased, pointing to disruption of trace element homeostasis. CONCLUSIONSSub-acute inhalation exposure of CuO NPs induced pulmonary inflammation, which was correlated to Cu concentrations in the lungs and started to resolve once exposure ended. Dosimetry analysis showed that Cu in the lungs was translocated into the bloodstream and heart tissue. Secondary organs affected by CuO NPs exposure were kidneys and spleen as they showed the disruption of trace element homeostasis and organ weight changes.
Details
- Title: Subtitle
- Time course of pulmonary inflammation and trace element biodistribution during and after sub-acute inhalation exposure to copper oxide nanoparticles in a murine model
- Creators
- Sudartip AreecheewakulAndrea Adamcakova-DoddEzazul HaqueXuefang JingDavid K MeyerholzPatrick T O'ShaughnessyPeter S ThorneAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- Particle and fibre toxicology, Vol.19(1), pp.40-40
- DOI
- 10.1186/s12989-022-00480-z
- PMID
- 35698146
- PMCID
- PMC9195454
- NLM abbreviation
- Part Fibre Toxicol
- eISSN
- 1743-8977
- Grant note
- DOI: 10.13039/100000009, name: Foundation for the National Institutes of Health, award: U01ES027252; DOI: 10.13039/100000002, name: National Institutes of Health, award: P30 ES005605
- Language
- English
- Date published
- 06/13/2022
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics; Pathology; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9984267160302771
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