Journal article
Tissue-Culture Assay of Antibodies to Heat-Labile Escherichia coli Enterotoxins
The New England journal of medicine, Vol.291(3), pp.117-121
07/18/1974
DOI: 10.1056/NEJM197407182910302
PMID: 4600168
Abstract
Serums of volunteers challenged orally with either toxigenic or invasive strains of
Escherichia coli
were analyzed for antibody content by means of an adrenal tumor-cell tissue-culture system and by the standard rabbit ileal-loop method. The tissue-culture assay system depends on the ability of specific antibody to neutralize the morphogenic effects of heat-labile
Esch. coli
enterotoxins. Six of seven volunteers receiving the toxigenic strains demonstrated fourfold or greater increases in antibody titers to heat-labile
Esch. coli
enterotoxins by the tissue-culture method, whereas eight of nine receiving the invasive
Esch. coli
strains did not demonstrate any such increases. These results compared favorably with those obtained by the ileal-loop method.
The adrenal cell morphologic assay method can be used in seroepidemiologic and clinical studies designed to assess the importance of heat-labile enterotoxigenic
Esch. coli
diarrheal disease. (N Engl J Med 291:117–121, 1974)
ENTEROTOXIGENIC
Escherichia coli
are probably the cause of many cases of diarrhea, not only in underdeveloped countries and other parts of the world but in the United States as well.
1
2
3
Other strains of
Esch. coli
that are not enterotoxigenic but possess invasive properties have also been implicated in diarrheal disease.
4
,
5
Two types of
Esch. coli
enterotoxin have been described, and both may be important in enteric disease of human beings and animals.
6
7
8
One toxin is of low molecular weight, is heat stable and effects changes in intestinal secretion that begin rapidly after exposure of small bowel to the toxin
9
,
10
; . . .
Details
- Title: Subtitle
- Tissue-Culture Assay of Antibodies to Heat-Labile Escherichia coli Enterotoxins
- Creators
- Sam T Donta - University of IowaDavid A Sack - University of IowaR. Bradley SackRobert B Wallace - University of IowaHerbert L Dupont - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The New England journal of medicine, Vol.291(3), pp.117-121
- DOI
- 10.1056/NEJM197407182910302
- PMID
- 4600168
- NLM abbreviation
- N Engl J Med
- ISSN
- 0028-4793
- eISSN
- 1533-4406
- Publisher
- Massachusetts Medical Society
- Language
- English
- Date published
- 07/18/1974
- Academic Unit
- Epidemiology; Injury Prevention Research Center; Internal Medicine
- Record Identifier
- 9984363639602771
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