Tissue Distribution, Metabolism, and Excretion of 3,3'-Dichloro-4'-sulfooxy-biphenyl in the Rat

Fabian A Grimm; Xianran He; Lynn M Teesch; Hans-Joachim Lehmler; Larry W Robertson; Michael W Duffel

doi:10.1021/acs.est.5b01499

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Tissue Distribution, Metabolism, and Excretion of 3,3'-Dichloro-4'-sulfooxy-biphenyl in the Rat

Journal article

Peer reviewed

Tissue Distribution, Metabolism, and Excretion of 3,3'-Dichloro-4'-sulfooxy-biphenyl in the Rat

Fabian A Grimm, Xianran He, Lynn M Teesch, Hans-Joachim Lehmler, Larry W Robertson and Michael W Duffel

Environmental science & technology, Vol.49(13), pp.8087-8095

07/07/2015

DOI: 10.1021/acs.est.5b01499

PMCID: PMC4496304

PMID: 26046945

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Abstract

Polychlorinated biphenyls (PCBs) with less chlorine atoms exhibit a greater susceptibility to metabolism than their more-chlorinated counterparts. Following initial hydroxylation of these less-chlorinated PCBs, metabolic sulfation to form PCB sulfates is increasingly recognized as an important component of their toxicology. Because procedures for the quantitative analysis of PCB sulfates in tissue samples have not been previously available, we have now developed an efficient, LC-ESI-MS/MS-based protocol for the quantitative analysis of 4-PCB 11 sulfate in biological samples. This procedure was used to determine the distribution of 4-PCB 11 sulfate in liver, kidney, lung, and brain as well as its excretion profile following its intravenous administration to male Sprague-Dawley rats. Following initial uptake of 4-PCB 11 sulfate, its concentration in these tissues and serum declined within the first hour following injection. Although biliary secretion was detected, analysis of 24 h collections of urine and feces revealed recovery of less than 4% of the administered 4-PCB 11 sulfate. High-resolution LC-MS analysis of bile, urine, and feces showed metabolic products derived from 4-PCB 11 sulfate. Thus, 4-PCB 11 sulfate at this dose was not directly excreted in the urine but was instead redistributed to tissues and/or subjected to further metabolism.

Tissue Distribution

Animals

Injections, Intravenous

Polychlorinated Biphenyls - chemistry

Spectrometry, Mass, Electrospray Ionization

Bile - chemistry

Chromatography, Liquid

Bile - metabolism

Male

Polychlorinated Biphenyls - metabolism

Polychlorinated Biphenyls - isolation & purification

Rats, Sprague-Dawley

ISRP Project 3 2015-2020

ISRP Project 1 2015-2020

Synthesis Core

Details

Title: Subtitle: Tissue Distribution, Metabolism, and Excretion of 3,3'-Dichloro-4'-sulfooxy-biphenyl in the Rat
Creators: Fabian A Grimm - †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States
Xianran He - †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States
Lynn M Teesch - †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States
Hans-Joachim Lehmler - †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States
Larry W Robertson - †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States
Michael W Duffel - †Interdisciplinary Graduate Program in Human Toxicology, ‡Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, §Department of Occupational and Environmental Health, College of Public Health, and ∥High Resolution Mass Spectrometry Facility, The University of Iowa, Iowa City, Iowa 52246, United States
Resource Type: Journal article
Publication Details: Environmental science & technology, Vol.49(13), pp.8087-8095
DOI: 10.1021/acs.est.5b01499
PMID: 26046945
PMCID: PMC4496304
NLM abbreviation: Environ Sci Technol
ISSN: 0013-936X
eISSN: 1520-5851
Publisher: United States
Grant note: P42 ES013661 / NIEHS NIH HHS P30 ES005605 / NIEHS NIH HHS P30 ES05605 / NIEHS NIH HHS
Language: English
Date published: 07/07/2015
Academic Unit: Occupational and Environmental Health; Core Research Facilities; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Iowa Superfund Research Program; Medicine Administration; Medicinal and Natural Products Chemistry
Record Identifier: 9984001086402771

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