Journal article
Tissue Engineering Strategies for Immature Teeth with Apical Periodontitis
Journal of endodontics, Vol.37(3), pp.390-397
2011
DOI: 10.1016/j.joen.2010.11.010
PMID: 21329828
Abstract
Regenerative endodontic treatment on immature teeth with apical periodontitis is promising but still not well-established. The purpose of this study was to explore novel strategies to engineer a vital support structure within a root canal space by a combination of induced blood clot, exposure of dentin matrix, and a cross-linked collagen scaffold.
Apical periodontitis was induced in 6 dogs with immature teeth (
n = 64). After disinfection, the following groups were randomly assigned: blood clot (BC) alone, BC with a cross-linked collagen scaffold (CCS), BC with exposure of dentin matrix by ethylenediaminetetraacetic acid (EDTA), and BC with CCS and EDTA. Positive (infected only) and negative controls (untreated) were also included. The dogs were followed up for 3.5 months and killed. Periradicular healing and root wall thickening were radiographically analyzed and statistically evaluated. The jaws were then fixed, demineralized, and subjected to histologic analyses. Newly formed mineralized tissues were histomorphometrically analyzed, quantified, and statistically evaluated.
Radiographically there was significant difference in periradicular healing and root wall thickening (
P < .05). Histomorphometric analysis showed significantly more mineralized tissue formation in the groups containing the scaffold (
P < .05). Exposure of the dentin matrix by EDTA appeared to increase the adherence of the newly formed mineralized tissue to the root walls.
The use of cross-linked collagen scaffold and exposure of dentin matrix combined with blood clot might provide an efficient approach to generate a vital support structure for the treatment of immature teeth with apical periodontitis.
Details
- Title: Subtitle
- Tissue Engineering Strategies for Immature Teeth with Apical Periodontitis
- Creators
- Nozomu Yamauchi - Department of Endodontics, University of North Carolina School of Dentistry, Chapel Hill, North CarolinaShizuko Yamauchi - Department of Endodontics, University of North Carolina School of Dentistry, Chapel Hill, North CarolinaHideaki Nagaoka - NC Oral Health Institute, University of North Carolina School of Dentistry, Chapel Hill, North CarolinaDerek Duggan - Department of Endodontics, University of North Carolina School of Dentistry, Chapel Hill, North CarolinaSheng Zhong - Department of Endodontics, University of North Carolina School of Dentistry, Chapel Hill, North CarolinaSun Min Lee - NC Oral Health Institute, University of North Carolina School of Dentistry, Chapel Hill, North CarolinaFabricio B Teixeira - Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio, TexasMitsuo Yamauchi - NC Oral Health Institute, University of North Carolina School of Dentistry, Chapel Hill, North Carolina
- Resource Type
- Journal article
- Publication Details
- Journal of endodontics, Vol.37(3), pp.390-397
- DOI
- 10.1016/j.joen.2010.11.010
- PMID
- 21329828
- NLM abbreviation
- J Endod
- ISSN
- 0099-2399
- eISSN
- 1878-3554
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2011
- Academic Unit
- Endodontics
- Record Identifier
- 9984065802702771
Metrics
24 Record Views