Journal article
Tissue-Specific Gene Expression Identifies a Gene in the Lysogenic Phage Gifsy-1 That Affects Salmonella enterica Serovar Typhimurium Survival in Peyer's Patches
Journal of bacteriology, Vol.182(16), pp.4406-4413
08/2000
DOI: 10.1128/JB.182.16.4406-4413.2000
PMCID: PMC94610
PMID: 10913072
Abstract
In vivo expression technology was used to identify
Salmonella enterica
serovar Typhimurium genes that are transcriptionally induced when the bacteria colonize the small intestines of mice. These genes were subsequently screened for those that are transcriptionally inactive during the systemic stages of disease. This procedure identified
gipA
, a gene that is specifically induced in the small intestine of the animal. The
gipA
gene is carried on the lambdoid phage Gifsy-1. Consistent with the expression profile, the sole defect conferred by a
gipA
null mutation is in growth or survival in a Peyer's patch. The
gipA
strain is wild type in its ability to initially colonize the small intestine and invade the intestinal epithelium. The mutant also survives and propagates at wild-type levels during the systemic stages of disease. The
gipA
open reading frame is homologous to a family of putative insertion sequence elements, although our evidence shows that transposition is not required for
gipA
function in the Peyer's patch. These results suggest that the bacteria sense and respond to the particular environment of the Peyer's patch, a critical site for the replication of
Salmonella
serovar Typhimurium.
Details
- Title: Subtitle
- Tissue-Specific Gene Expression Identifies a Gene in the Lysogenic Phage Gifsy-1 That Affects Salmonella enterica Serovar Typhimurium Survival in Peyer's Patches
- Creators
- Theresa L Stanley - Department of Microbiology, University of Illinois, Urbana, Illinois 61801Craig D Ellermeier - Department of Microbiology, University of Illinois, Urbana, Illinois 61801James M Slauch - Department of Microbiology, University of Illinois, Urbana, Illinois 61801
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.182(16), pp.4406-4413
- DOI
- 10.1128/JB.182.16.4406-4413.2000
- PMID
- 10913072
- PMCID
- PMC94610
- NLM abbreviation
- J Bacteriol
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 08/2000
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083840702771
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