Journal article
Tissue-based membrane electrode with high biocatalytic activity for measurement of adenosine 5'-monophosphate
Analytical chemistry (Washington), Vol.53(12), pp.1837-1842
10/1981
DOI: 10.1021/ac00235a027
PMID: 7294355
Abstract
A bloselectlve membrane electrode which employs slices of rabbit muscle tissue held at an ammonia gas sensor Is shown to have nearly 50 times greater biocatalytic activity for adenosine 5'-monophosphate (AMP) than conventional electrodes using commercially available enzyme preparations. As a consequence of this high specific activity, enzyme concentration steps are eliminated and the rabbit muscle electrode achieves excellent response characteristics with a working lifetime of at least 28 days. Important experimental variables, Including the effect of tissue thickness, are evaluated In order to optimize electrode response.
Details
- Title: Subtitle
- Tissue-based membrane electrode with high biocatalytic activity for measurement of adenosine 5'-monophosphate
- Creators
- M A Arnold - University of Iowa, Fraternal Order of Eagles Diabetes Research CenterG A Rechnitz
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.53(12), pp.1837-1842
- DOI
- 10.1021/ac00235a027
- PMID
- 7294355
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Language
- English
- Date published
- 10/1981
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216588602771
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