Journal article
Tissue-engineered Rabbit Cranial Suture from Autologous Fibroblasts and BMP2
Journal of dental research, Vol.83(10), pp.751-756
10/2004
DOI: 10.1177/154405910408301003
PMID: 15381713
Abstract
Craniosynostosis is a congenital disorder of premature ossification of cranial sutures, occurring in one of approximately every 2500 live human births. This work addressed a hypothesis that a cranial suture can be tissue-engineered from autologous cells. Dermal fibroblasts were isolated subcutaneously from growing rabbits, culture-expanded, and seeded in a gelatin scaffold. We fabricated a composite tissue construct by sandwiching the fibroblast-seeded gelatin scaffold between two collagen sponges loaded with recombinant human BMP2. Surgically created, full-thickness parietal defects were filled with the composite tissue construct in the same rabbits from which dermal fibroblasts had been obtained. After four-week in vivo implantation, there was de novo formation of tissue-engineered cranial suture, microscopically reminiscent of the adjacent natural cranial suture. The tissue-engineered cranial suture showed radiolucency on radiographic images, in contrast to radio-opacity of microscopically ossified calvarial defects filled with fibroblast-free, BMP2-loaded constructs. This approach may be refined for tissue engineering of cranial sutures for craniosynostosis patients.
Details
- Title: Subtitle
- Tissue-engineered Rabbit Cranial Suture from Autologous Fibroblasts and BMP2
- Creators
- L. Hong - University of Illinois ChicagoJ.J. Mao - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.83(10), pp.751-756
- DOI
- 10.1177/154405910408301003
- PMID
- 15381713
- NLM abbreviation
- J Dent Res
- ISSN
- 0022-0345
- eISSN
- 1544-0591
- Language
- English
- Date published
- 10/2004
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367749202771
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