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Tissue-resident CCR2+ macrophage TREM-1/3 signaling is necessary for monocyte and neutrophil recruitment to injured hearts
Journal article   Peer reviewed

Tissue-resident CCR2+ macrophage TREM-1/3 signaling is necessary for monocyte and neutrophil recruitment to injured hearts

Yuriko Terada, Wenjun Li, Junedh M. Amrute, Amit I. Bery, Charles R. Liu, Venkatrao Nunna, Christian Corbin Frye, Hao Dun, Andrew L. Koenig, Hannah P. Luehmann, …
Cell reports (Cambridge), Vol.44(3), p.115380
03/25/2025
DOI: 10.1016/j.celrep.2025.115380
PMID: 40042972
url
https://doi.org/10.1016/j.celrep.2025.115380View
Published (Version of record) Open Access

Abstract

Triggering receptor expressed on myeloid cells 1 (TREM-1) has been shown to amplify inflammatory signals, such as Toll-like receptor signaling, after infection and sterile injury. While previous studies have demonstrated that TREM-1 activation in circulating immune cells promotes injury, the role of TREM-1 signaling in tissue-resident cells in the context of sterile inflammation remains poorly understood. Here, we used a cardiac transplantation model to dissect how Trem1/3 expression on heart-resident cells regulates sterile inflammation. TREM-1 is expressed in heart-resident C-C chemokine receptor 2 (CCR2)+ macrophages in mice and humans. TREM-1/3 signaling in tissue-resident CCR2+ macrophages promotes C-C motif chemokine ligand 3 (CCL3) production and is critical for recruiting neutrophils and CCR2+ monocytes after heart transplantation. We demonstrate prolonged allograft survival after transplantation of Trem1/3-deficient compared with wild-type hearts. We identify TREM-1/3 signaling in donor grafts as a potential future therapeutic target to blunt inflammation after myocardial ischemia-reperfusion injury. [Display omitted] •Trem1/3 expression in CCR2+ cardiac macrophages drives production of inflammatory chemokines•Neutrophil/monocyte recruitment to transplanted hearts depends on graft Trem1/3 expression•Graft Trem1/3 expression enhances alloimmunity after heart transplantation Outcomes after heart transplantation are limited by ischemia-reperfusion injury, a process driven by infiltration of immune cells. Terada et al. identify TREM-1/3 signaling in graft-resident CCR2+ macrophages as a critical driver of immune cell infiltration, graft injury, and rejection after heart transplantation.
CCR2 heart macrophages transplantation TREM-1/3

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