Tissue specificity and cellular distribution of Novikoff hepatoma antigenic proteins p39, p49, and p56

Warren N Schmidt; Marta Stryjecka-Zimmer; William F Glass II; Robert C Briggs; Lubomir S Hnilica

doi:10.1016/S0021-9258(18)43396-0

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Tissue specificity and cellular distribution of Novikoff hepatoma antigenic proteins p39, p49, and p56

Journal article

Open access

Peer reviewed

Tissue specificity and cellular distribution of Novikoff hepatoma antigenic proteins p39, p49, and p56

Warren N Schmidt, Marta Stryjecka-Zimmer, William F Glass II, Robert C Briggs and Lubomir S Hnilica

The Journal of biological chemistry, Vol.256(15), pp.8117-8123

08/10/1981

DOI: 10.1016/S0021-9258(18)43396-0

PMID: 7263643

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Abstract

Three Novikoff hepatoma protein antigens (approximately Mr = 39,000, 49,000, and 56,000) were partially purified from Novikoff hepatoma chromatin. Rabbit antiserum to these three proteins was used to examine various rat tissues for the presence of these antigens. Immunological specificity of the antiserum was assessed using quantitative microcomplement fixation assay or by visualization of the immunoreactive complexes with peroxidase-antiperoxidase procedure after transferring the electrophoretically separated proteins to nitrocellulose sheets. The immunoreactivity was localized with the three proteins p39, p49, and p56 in Novikoff hepatoma. The p56 protein was found to be present in normal rat liver, 24-h regenerating rat liver, fetal rat liver, or kidney, albeit in much smaller amounts as found in Novikoff hepatoma. the p49 and p39 antigens were specific for Novikoff hepatoma. Immunoabsorption experiments confirmed the specificity of this antiserum. Assessment of various subcellular fractions of Novikoff hepatoma revealed that the p39, p49, and p56 protein antigens are present in the cytoplasmic fractions as well as in isolated chromatin.

Fetus

Cell Nucleus - immunology

Liver Regeneration

Kidney - immunology

Rats

Complement Fixation Tests

Male

Liver Neoplasms, Experimental - immunology

Tissue Distribution

Liver - immunology

Animals

Chromatin - immunology

Neoplasm Proteins - analysis

Antigens, Neoplasm - analysis

Details

Title: Subtitle: Tissue specificity and cellular distribution of Novikoff hepatoma antigenic proteins p39, p49, and p56
Creators: Warren N Schmidt
Marta Stryjecka-Zimmer
William F Glass II
Robert C Briggs
Lubomir S Hnilica
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.256(15), pp.8117-8123
DOI: 10.1016/S0021-9258(18)43396-0
PMID: 7263643
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Grant note: CA-26412 / NCI NIH HHS
Language: English
Date published: 08/10/1981
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984094510802771

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