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Tobacco Xenobiotics Release Nitric Oxide
Journal article   Open access   Peer reviewed

Tobacco Xenobiotics Release Nitric Oxide

EWN Lam, E E Kelley, S M Martin and G R Buettner
Tobacco induced diseases, Vol.1(3), pp.207-211
09/15/2003
DOI: 10.1186/1617-9625-1-3-207
PMID: 19570261
url
https://doi.org/10.1186/1617-9625-1-3-207View
Published (Version of record) Open Access

Abstract

Many xenobiotic compounds exert their actions through the release of free radicals and related oxidants [ 1 , 2 ], bringing about unwanted biological effects [ 3 ]. Indeed, oxidative events may play a significant role in tobacco toxicity from cigarette smoke. Here, we demonstrate the direct in vitro release of the free radical nitric oxide ( • NO) from extracts and components of smokeless tobacco, including nicotine, nitrosonornicotine (NNN) and 4-(methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in phosphate buffered saline and human saliva using electron spin resonance and chemiluminescence detection. Our findings suggest that tobacco xenobiotics represent as yet unrecognized sources of • NO in the body.
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