Journal article
Tolerance of oocyte plasma membrane to electric current changes after fertilisation
Zygote (Cambridge), Vol.4(4), pp.275-278
11/1996
DOI: 10.1017/S0967199400003221
PMID: 9153765
Abstract
The tolerance of the oocyte plasma membrane (oolemma) to electrical pulses (TEP) was investigated for oocytes, zygotes, and embryos at the early and late 2-cell stage. The oocyte survival rate after two electrical pulses (1.4KV/cm for 640μs each) was used as an indicator of the TEP of the oolemma. Survival rate of mid-pronuclear zygotes (94.3%±2.3%) was significantly (p < 0.05) higher than that of recently ovulated (2.1%±1.9%) and in vivo aged (25.1%±2.6%) oocytes; survival rate of in vivo aged oocytes was also significantly higher than that of recently ovulated oocytes. Soon after fertilisation, the survival rate of the oocytes markedly increased, up to 94% at the mid-pronuclear stage. Survival ratedropped thereafter. These results suggest that the characteristics of the oocyte plasma membrane (oolemma) change after fertilisation.
Details
- Title: Subtitle
- Tolerance of oocyte plasma membrane to electric current changes after fertilisation
- Creators
- Tan Jing-he - Department of Animal science, Northeat Agricultural University, Harbin, ChinaLiu Zhong-hua - Department of Animal science, Northeat Agricultural University, Harbin, ChinaSun Xing-shen - Department of Animal science, Northeat Agricultural University, Harbin, ChinaHe Gui-xin - Department of Animal science, Northeat Agricultural University, Harbin, China
- Resource Type
- Journal article
- Publication Details
- Zygote (Cambridge), Vol.4(4), pp.275-278
- Publisher
- Cambridge University Press
- DOI
- 10.1017/S0967199400003221
- PMID
- 9153765
- ISSN
- 0967-1994
- eISSN
- 1469-8730
- Number of pages
- 4
- Language
- English
- Date published
- 11/1996
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284350802771
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