Journal article
Topical Treatment with Liposomes Containing T4 Endonuclease V Protects Human Skin In Vivo from Ultraviolet-Induced Upregulation of Interleukin-10 and Tumor Necrosis Factor-α
Journal of investigative dermatology, Vol.114(1), pp.149-156
01/2000
DOI: 10.1046/j.1523-1747.2000.00839.x
PMID: 10620131
Abstract
Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-α RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.
Details
- Title: Subtitle
- Topical Treatment with Liposomes Containing T4 Endonuclease V Protects Human Skin In Vivo from Ultraviolet-Induced Upregulation of Interleukin-10 and Tumor Necrosis Factor-α
- Creators
- Peter WolfRobert R MülleggerH Peter SoyerAngelika HoferJosef SmolleMichael HornLorenzo CerroniRainer Hofmann-WellenhofHelmut KerlHarald Maier - Division of Special and Environmental Dermatology, Department of Dermatology, University of Vienna, Vienna, AustriaCaroline A Chadwick - CRC Epithelial Biology Group, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, U.KDaniel Yarosh - Applied Genetics Inc. Dermatics, Freeport, New York, New York, U.S.AJonathan Klein - Applied Genetics Inc. Dermatics, Freeport, New York, New York, U.S.ACorazon Bucana - Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, Texas, U.S.AKenneth Dunner - Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, Texas, U.S.AChristopher S Potten - CRC Epithelial Biology Group, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, U.KHerbert Hönigsmann - Division of Special and Environmental Dermatology, Department of Dermatology, University of Vienna, Vienna, AustriaMargaret L Kripke - Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, Texas, U.S.A
- Resource Type
- Journal article
- Publication Details
- Journal of investigative dermatology, Vol.114(1), pp.149-156
- Publisher
- Elsevier Inc
- DOI
- 10.1046/j.1523-1747.2000.00839.x
- PMID
- 10620131
- ISSN
- 0022-202X
- eISSN
- 1523-1747
- Language
- English
- Date published
- 01/2000
- Academic Unit
- Stead Family Department of Pediatrics; Neonatology
- Record Identifier
- 9984093325002771
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