Journal article
Topoisomerase II-mediated DNA damage of episomes in tumor-bearing mice
Cancer research (Chicago, Ill.), Vol.50(19), pp.6154-6157
10/01/1990
PMID: 2169334
Abstract
Observations of cells in culture have demonstrated that, for many antitumor agents, topoisomerase II-mediated DNA damage relates to cytotoxicity. However, there is no evidence in tumor-bearing animals to suggest that such agents induce topoisomerase II-mediated damage of DNA in solid tumors or that such damage reflects inhibition of tumor growth. To address this question, a mouse fibroblast cell line neoplastically transformed by an episomal element containing the v-Ha-ras and bovine papillomavirus genes was utilized to measure topoisomerase II-induced DNA damage and growth inhibition of solid tumors derived from this line. Using the topoisomerase II inhibitor amsacrine, the episomal element was found to be a sensitive indicator of topoisomerase II-mediated damage in vivo. The DNA breaks induced by single i.v. injections of amsacrine were protein linked and occurred preferentially in episomal regulatory regions. A strong correlation between suppression of tumor growth and topoisomerase II-mediated damage of the episome was demonstrated.
Details
- Title: Subtitle
- Topoisomerase II-mediated DNA damage of episomes in tumor-bearing mice
- Creators
- E B CullinanL S GawronY M RustumT A Beerman
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.50(19), pp.6154-6157
- PMID
- 2169334
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 10/01/1990
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359776002771
Metrics
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